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dc.contributor.authorTarnow, Inge
dc.contributor.authorKristensen, Annemarie T.
dc.contributor.authorKrogh, Anne K.R.
dc.contributor.authorFrelinger, Andrew L. III
dc.contributor.authorBarnard, Marc R.
dc.contributor.authorMichelson, Alan D.
dc.date2022-08-11T08:10:10.000
dc.date.accessioned2022-08-23T16:58:07Z
dc.date.available2022-08-23T16:58:07Z
dc.date.issued2008-06-01
dc.date.submitted2012-04-25
dc.identifier.citationVet Immunol Immunopathol. 2008 Jun 15;123(3-4):345-52. Epub 2008 Mar 4. doi 10.1016/j.vetimm.2008.02.016
dc.identifier.issn0165-2427 (Linking)
dc.identifier.doi10.1016/j.vetimm.2008.02.016
dc.identifier.pmid18405981
dc.identifier.urihttp://hdl.handle.net/20.500.14038/43298
dc.description.abstractPlatelets play a role in both the innate and adaptive immune systems. Methods for detecting activated platelets and leukocyte-platelet aggregates (LPAs) are useful for basic and applied research concerning the role of platelets in inflammation and immune disorders. The aim of the study was to develop flow cytometric assays for detection of platelets binding to monocytes and neutrophils and for activated platelets in canine whole blood and to investigate the effect of physiologic agonists. Citrate anticoagulated whole blood was incubated with monoclonal antibodies against CD14 and CD61 for detection of LPAs, and the effect of various agonists was investigated. For detection of activated platelets, whole blood was incubated with monoclonal antibodies against CD62P and against a receptor-induced binding site on fibrinogen (CAP1) with CD61 as a platelet identifier. Isotype controls were prepared in parallel. The individual physiologic agonists ADP, collagen and epinephrine increased LPAs, CD62P and CAP1 binding only modestly. However, combinations of agonists gave more substantial increases. A dose-response relationship was seen using alpha- and gamma-thrombin, and ADP as agonists. In conclusion, we have developed flow cytometry assays to measure LPAs and platelet activation in canine whole blood, and have explored the effect of various physiologic agonists at different concentrations.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=18405981&dopt=Abstract">Link to article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1016/j.vetimm.2008.02.016
dc.subjectAdenosine Diphosphate
dc.subjectAnimals
dc.subjectAntibodies, Monoclonal
dc.subjectAntigens, CD14
dc.subjectBlood Platelets
dc.subjectCollagen
dc.subjectDogs
dc.subjectEpinephrine
dc.subjectFlow Cytometry
dc.subjectIntegrin beta3
dc.subjectLeukocytes, Mononuclear
dc.subjectNeutrophils
dc.subjectP-Selectin
dc.subjectPlatelet Aggregation
dc.subjectThrombin
dc.subjectHematology
dc.subjectOncology
dc.subjectPediatrics
dc.titleEffects of physiologic agonists on canine whole blood flow cytometry assays of leukocyte-platelet aggregation and platelet activation
dc.typeJournal Article
dc.source.journaltitleVeterinary immunology and immunopathology
dc.source.volume123
dc.source.issue3-4
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/peds_hematology/102
dc.identifier.contextkey2796592
html.description.abstract<p>Platelets play a role in both the innate and adaptive immune systems. Methods for detecting activated platelets and leukocyte-platelet aggregates (LPAs) are useful for basic and applied research concerning the role of platelets in inflammation and immune disorders. The aim of the study was to develop flow cytometric assays for detection of platelets binding to monocytes and neutrophils and for activated platelets in canine whole blood and to investigate the effect of physiologic agonists. Citrate anticoagulated whole blood was incubated with monoclonal antibodies against CD14 and CD61 for detection of LPAs, and the effect of various agonists was investigated. For detection of activated platelets, whole blood was incubated with monoclonal antibodies against CD62P and against a receptor-induced binding site on fibrinogen (CAP1) with CD61 as a platelet identifier. Isotype controls were prepared in parallel. The individual physiologic agonists ADP, collagen and epinephrine increased LPAs, CD62P and CAP1 binding only modestly. However, combinations of agonists gave more substantial increases. A dose-response relationship was seen using alpha- and gamma-thrombin, and ADP as agonists. In conclusion, we have developed flow cytometry assays to measure LPAs and platelet activation in canine whole blood, and have explored the effect of various physiologic agonists at different concentrations.</p>
dc.identifier.submissionpathpeds_hematology/102
dc.contributor.departmentDepartment of Pediatrics
dc.source.pages345-52


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