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dc.contributor.authorYasukochi, Yukio
dc.contributor.authorMaruyama, Osamu
dc.contributor.authorMahajan, Milind C.
dc.contributor.authorPadden, Carolyn
dc.contributor.authorEuskirchen, Ghia M.
dc.contributor.authorSchulz, Vincent
dc.contributor.authorHirakawa, Hideki
dc.contributor.authorKuhara, Satoru
dc.contributor.authorPan, Xing-Hua
dc.contributor.authorNewburger, Peter E.
dc.contributor.authorSnyder, Michael
dc.contributor.authorWeissman, Sherman M.
dc.date2022-08-11T08:10:10.000
dc.date.accessioned2022-08-23T16:58:14Z
dc.date.available2022-08-23T16:58:14Z
dc.date.issued2010-02-23
dc.date.submitted2012-04-25
dc.identifier.citationProc Natl Acad Sci U S A. 2010 Feb 23;107(8):3704-9. Epub 2010 Feb 2. <a href="http://dx.doi.org/10.1073/pnas.0914812107">Link to article on publisher's website</a>
dc.identifier.issn0027-8424 (Linking)
dc.identifier.doi10.1073/pnas.0914812107
dc.identifier.pmid20133578
dc.identifier.urihttp://hdl.handle.net/20.500.14038/43325
dc.description.abstractThe DNA methylation status of human X chromosomes from male and female neutrophils was identified by high-throughput sequencing of HpaII and MspI digested fragments. In the intergenic and intragenic regions on the X chromosome, the sites outside CpG islands were heavily hypermethylated to the same degree in both genders. Nearly half of X chromosome promoters were either hypomethylated or hypermethylated in both females and males. Nearly one third of X chromosome promoters were a mixture of hypomethylated and heterogeneously methylated sites in females and were hypomethylated in males. Thus, a large fraction of genes that are silenced on the inactive X chromosome are hypomethylated in their promoter regions. These genes frequently belong to the evolutionarily younger strata of the X chromosome. The promoters that were hypomethylated at more than two sites contained most of the genes that escaped silencing on the inactive X chromosome. The overall levels of expression of X-linked genes were indistinguishable in females and males, regardless of the methylation state of the inactive X chromosome. Thus, in addition to DNA methylation, other factors are involved in the fine tuning of gene dosage compensation in neutrophils.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=20133578&dopt=Abstract">Link to article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1073/pnas.0914812107
dc.rights<p>Publisher PDF posted as allowed by the publisher's author rights policy at http://www.pnas.org/site/misc/authorfaq.shtml.</p>
dc.subjectChromosomes, Human, X
dc.subject*DNA Methylation
dc.subjectFemale
dc.subject*Gene Expression
dc.subject*Gene Expression Regulation
dc.subject*Genes, X-Linked
dc.subjectHumans
dc.subjectMale
dc.subjectNeutrophils
dc.subjectPromoter Regions, Genetic
dc.subjectSex Factors
dc.subjectHematology
dc.subjectOncology
dc.subjectPediatrics
dc.titleX chromosome-wide analyses of genomic DNA methylation states and gene expression in male and female neutrophils
dc.typeJournal Article
dc.source.journaltitleProceedings of the National Academy of Sciences of the United States of America
dc.source.volume107
dc.source.issue8
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=1127&amp;context=peds_hematology&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/peds_hematology/128
dc.identifier.contextkey2796618
refterms.dateFOA2022-08-23T16:58:14Z
html.description.abstract<p>The DNA methylation status of human X chromosomes from male and female neutrophils was identified by high-throughput sequencing of HpaII and MspI digested fragments. In the intergenic and intragenic regions on the X chromosome, the sites outside CpG islands were heavily hypermethylated to the same degree in both genders. Nearly half of X chromosome promoters were either hypomethylated or hypermethylated in both females and males. Nearly one third of X chromosome promoters were a mixture of hypomethylated and heterogeneously methylated sites in females and were hypomethylated in males. Thus, a large fraction of genes that are silenced on the inactive X chromosome are hypomethylated in their promoter regions. These genes frequently belong to the evolutionarily younger strata of the X chromosome. The promoters that were hypomethylated at more than two sites contained most of the genes that escaped silencing on the inactive X chromosome. The overall levels of expression of X-linked genes were indistinguishable in females and males, regardless of the methylation state of the inactive X chromosome. Thus, in addition to DNA methylation, other factors are involved in the fine tuning of gene dosage compensation in neutrophils.</p>
dc.identifier.submissionpathpeds_hematology/128
dc.contributor.departmentDepartment of Pediatrics
dc.source.pages3704-9


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