AuthorsShearstone, Jeffrey R.
UMass Chan AffiliationsDepartment of Cancer Biology
Department of Pediatrics
Document TypeJournal Article
Gene Expression Regulation, Developmental
Locus Control Region
Long Interspersed Nucleotide Elements
Sequence Analysis, DNA
MetadataShow full item record
AbstractIn the mammalian genome, 5'-CpG-3' dinucleotides are frequently methylated, correlating with transcriptional silencing. Genome-wide demethylation is thought to occur only twice during development, in primordial germ cells and in the pre-implantation embryo. These demethylation events are followed by de novo methylation, setting up a pattern inherited throughout development and modified only at tissue-specific loci. We studied DNA methylation in differentiating mouse erythroblasts in vivo by using genomic-scale reduced representation bisulfite sequencing (RRBS). Demethylation at the erythroid-specific β-globin locus was coincident with global DNA demethylation at most genomic elements. Global demethylation was continuous throughout differentiation and required rapid DNA replication. Hence, DNA demethylation can occur globally during somatic cell differentiation, providing an experimental model for its study in development and disease.
SourceScience. 2011 Nov 11;334(6057):799-802. DOI: 10.1126/science.1207306. Link to article on publisher's website
Permanent Link to this Itemhttp://hdl.handle.net/20.500.14038/43333
Related ResourcesLink to article in PubMed