Authors
Shearstone, Jeffrey R.Pop, Ramona
Bock, Christoph
Boyle, Patrick
Meissner, Alexander
Socolovsky, Merav
Document Type
Journal ArticlePublication Date
2011-11-11
Metadata
Show full item recordAbstract
In the mammalian genome, 5'-CpG-3' dinucleotides are frequently methylated, correlating with transcriptional silencing. Genome-wide demethylation is thought to occur only twice during development, in primordial germ cells and in the pre-implantation embryo. These demethylation events are followed by de novo methylation, setting up a pattern inherited throughout development and modified only at tissue-specific loci. We studied DNA methylation in differentiating mouse erythroblasts in vivo by using genomic-scale reduced representation bisulfite sequencing (RRBS). Demethylation at the erythroid-specific β-globin locus was coincident with global DNA demethylation at most genomic elements. Global demethylation was continuous throughout differentiation and required rapid DNA replication. Hence, DNA demethylation can occur globally during somatic cell differentiation, providing an experimental model for its study in development and disease.Source
Science. 2011 Nov 11;334(6057):799-802. DOI: 10.1126/science.1207306. Link to article on publisher's websiteDOI
10.1126/science.1207306Permanent Link to this Item
http://hdl.handle.net/20.500.14038/43333PubMed ID
22076376Related Resources
Link to article in PubMedae974a485f413a2113503eed53cd6c53
10.1126/science.1207306