Show simple item record

dc.contributor.authorBarnard, Marc R.
dc.contributor.authorMacgregor, Hollace
dc.contributor.authorRagno, G.
dc.contributor.authorPivacek, L. E.
dc.contributor.authorKhuri, Shukri F.
dc.contributor.authorMichelson, Alan D.
dc.contributor.authorValeri, C. Robert
dc.date2022-08-11T08:10:10.000
dc.date.accessioned2022-08-23T16:58:19Z
dc.date.available2022-08-23T16:58:19Z
dc.date.issued1999-08-01
dc.date.submitted2012-04-25
dc.identifier.citationTransfusion. 1999 Aug;39(8):880-8. DOI: 10.1046/j.1537-2995.1999.39080880.x
dc.identifier.issn0041-1132 (Linking)
dc.identifier.doi10.1046/j.1537-2995.1999.39080880.x
dc.identifier.pmid10504125
dc.identifier.urihttp://hdl.handle.net/20.500.14038/43340
dc.description.abstractBACKGROUND: A study in humans showed that the transfusion of previously frozen human platelets after cardiopulmonary bypass, despite decreased survival, resulted in better hemostatic function than that of liquid-preserved platelets stored at 22 degrees C for 3 to 4 days. STUDY DESIGN AND METHODS: In this study, fresh, 3- to 4-day-old liquid-preserved, and cryopreserved human platelets were studied by the use of monoclonal antibodies directed against p-selectin, glycoprotein (GP)Ib, activated GPIIb/IIIa, and coagulation factor V in a three-color flow cytometric method. RESULTS: The fresh and liquid-preserved platelets had normal surface levels of GPIb, while the cryopreserved platelets were composed of distinct subpopulations of GPIb-normal and GPIb-reduced platelets. On the basis of the binding of factor V, both subpopulations of cryopreserved platelets exhibited greater surface binding of factor V than did fresh and liquid-preserved platelets. Activated GPIIb/IIIa was elevated on GPIb-normal platelets, but not on GPIb-reduced platelets. Baboon platelets frozen by a procedure identical to that used to freeze human platelets also had GPIb-normal and GPIb-reduced subpopulations after the freezing-thawing-washing procedure. Autologous cryopreserved baboon platelets labeled with biotin-X-N-hydroxysuccinimide showed a rapid removal of GPIb-reduced platelets during the 5-minute postinfusion period, whereas GPIb-normal platelets had an in vivo recovery of 48 percent and a lifespan of slightly less than 6 days. CONCLUSIONS: Improved in vivo function of cryopreserved platelets may be related to the rapid hemostatic effect of the GPIb-reduced subpopulation secondary to increased binding of factor V and expression of p-selectin.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=10504125&dopt=Abstract">Link to article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1046/j.1537-2995.1999.39080880.x
dc.subjectAnimals
dc.subjectAntibodies, Monoclonal
dc.subjectBiotin
dc.subject*Blood Platelets
dc.subject*Blood Preservation
dc.subjectCell Survival
dc.subject*Cryopreservation
dc.subjectFlow Cytometry
dc.subjectHumans
dc.subjectP-Selectin
dc.subjectPapio
dc.subjectPlatelet Glycoprotein GPIIb-IIIa Complex
dc.subjectPlatelet Glycoprotein GPIb-IX Complex
dc.subjectHematology
dc.subjectOncology
dc.subjectPediatrics
dc.titleFresh, liquid-preserved, and cryopreserved platelets: adhesive surface receptors and membrane procoagulant activity
dc.typeJournal Article
dc.source.journaltitleTransfusion
dc.source.volume39
dc.source.issue8
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/peds_hematology/19
dc.identifier.contextkey2796506
html.description.abstract<p>BACKGROUND: A study in humans showed that the transfusion of previously frozen human platelets after cardiopulmonary bypass, despite decreased survival, resulted in better hemostatic function than that of liquid-preserved platelets stored at 22 degrees C for 3 to 4 days. STUDY DESIGN AND</p> <p>METHODS: In this study, fresh, 3- to 4-day-old liquid-preserved, and cryopreserved human platelets were studied by the use of monoclonal antibodies directed against p-selectin, glycoprotein (GP)Ib, activated GPIIb/IIIa, and coagulation factor V in a three-color flow cytometric method.</p> <p>RESULTS: The fresh and liquid-preserved platelets had normal surface levels of GPIb, while the cryopreserved platelets were composed of distinct subpopulations of GPIb-normal and GPIb-reduced platelets. On the basis of the binding of factor V, both subpopulations of cryopreserved platelets exhibited greater surface binding of factor V than did fresh and liquid-preserved platelets. Activated GPIIb/IIIa was elevated on GPIb-normal platelets, but not on GPIb-reduced platelets. Baboon platelets frozen by a procedure identical to that used to freeze human platelets also had GPIb-normal and GPIb-reduced subpopulations after the freezing-thawing-washing procedure. Autologous cryopreserved baboon platelets labeled with biotin-X-N-hydroxysuccinimide showed a rapid removal of GPIb-reduced platelets during the 5-minute postinfusion period, whereas GPIb-normal platelets had an in vivo recovery of 48 percent and a lifespan of slightly less than 6 days.</p> <p>CONCLUSIONS: Improved in vivo function of cryopreserved platelets may be related to the rapid hemostatic effect of the GPIb-reduced subpopulation secondary to increased binding of factor V and expression of p-selectin.</p>
dc.identifier.submissionpathpeds_hematology/19
dc.contributor.departmentDepartment of Pediatrics
dc.source.pages880-8


This item appears in the following Collection(s)

Show simple item record