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dc.contributor.authorClaytor, R. Brannon
dc.contributor.authorLi, Jian-ming
dc.contributor.authorFurman, Mark I.
dc.contributor.authorGarnette, Charles S. C.
dc.contributor.authorRohrer, Michael J.
dc.contributor.authorBarnard, Marc R.
dc.contributor.authorKrueger, Lori A.
dc.contributor.authorFrelinger, Andrew L. III
dc.contributor.authorMichelson, Alan D.
dc.date2022-08-11T08:10:10.000
dc.date.accessioned2022-08-23T16:58:23Z
dc.date.available2022-08-23T16:58:23Z
dc.date.issued2001-04-01
dc.date.submitted2012-04-25
dc.identifier.citationCytometry. 2001 Apr 1;43(4):308-13.
dc.identifier.issn0196-4763 (Linking)
dc.identifier.pmid11260598
dc.identifier.urihttp://hdl.handle.net/20.500.14038/43355
dc.description.abstractBACKGROUND: Adherence of platelets to endothelial cells may be a significant event in the development of vascular thrombosis. Existing models, which examine platelet-endothelial cell interactions, compromise endothelial cell integrity or use radioactivity to identify platelets that adhere to endothelial cells. We report a novel method for in vitro detection of platelet-endothelial cell adhesion that allows endothelial cells to remain as an intact monolayer and for visualization of individual platelets. METHODS: Fluorescently labeled platelets were incubated with a confluent monolayer of endothelial cells. Laser scanning cytometry (LSC) identified platelets bound to endothelial cells based on their fluorescent signals. RESULTS: LSC detection of platelets reliably reproduced well-described findings of thrombin-induced platelet-endothelial cell adhesion. Results demonstrating reduced adhesion with a glycoprotein IIb-IIIa-specific blocking monoclonal antibody confirmed the specificity of the LSC detection of platelet-endothelial cell adhesion. CONCLUSIONS: LSC is a novel method for detecting platelet--endothelial cell adhesion. Its advantages over other methods are: (a) endothelial cells remain undisturbed and adherent throughout; (b) the ability to detect individual bound platelets and subpopulations; (c) the ability to store images and slides and then relocate, revisualize, and reanalyze individual cells or cell populations of interest; and (d) no radioactivity.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11260598&dopt=Abstract">Link to article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1002/1097-0320(20010401)43:4<308::AID-CYTO1063>3.0.CO;2-9
dc.subjectHematology
dc.subjectOncology
dc.subjectPediatrics
dc.titleLaser scanning cytometry: a novel method for the detection of platelet--endothelial cell adhesion
dc.typeJournal Article
dc.source.journaltitleCytometry
dc.source.volume43
dc.source.issue4
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/peds_hematology/33
dc.identifier.contextkey2796521
html.description.abstract<p>BACKGROUND: Adherence of platelets to endothelial cells may be a significant event in the development of vascular thrombosis. Existing models, which examine platelet-endothelial cell interactions, compromise endothelial cell integrity or use radioactivity to identify platelets that adhere to endothelial cells. We report a novel method for in vitro detection of platelet-endothelial cell adhesion that allows endothelial cells to remain as an intact monolayer and for visualization of individual platelets.</p> <p>METHODS: Fluorescently labeled platelets were incubated with a confluent monolayer of endothelial cells. Laser scanning cytometry (LSC) identified platelets bound to endothelial cells based on their fluorescent signals.</p> <p>RESULTS: LSC detection of platelets reliably reproduced well-described findings of thrombin-induced platelet-endothelial cell adhesion. Results demonstrating reduced adhesion with a glycoprotein IIb-IIIa-specific blocking monoclonal antibody confirmed the specificity of the LSC detection of platelet-endothelial cell adhesion.</p> <p>CONCLUSIONS: LSC is a novel method for detecting platelet--endothelial cell adhesion. Its advantages over other methods are: (a) endothelial cells remain undisturbed and adherent throughout; (b) the ability to detect individual bound platelets and subpopulations; (c) the ability to store images and slides and then relocate, revisualize, and reanalyze individual cells or cell populations of interest; and (d) no radioactivity.</p>
dc.identifier.submissionpathpeds_hematology/33
dc.contributor.departmentDepartment of Surgery
dc.contributor.departmentDepartment of Pediatrics
dc.source.pages308-13


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