Laser scanning cytometry: a novel method for the detection of platelet--endothelial cell adhesion
dc.contributor.author | Claytor, R. Brannon | |
dc.contributor.author | Li, Jian-ming | |
dc.contributor.author | Furman, Mark I. | |
dc.contributor.author | Garnette, Charles S. C. | |
dc.contributor.author | Rohrer, Michael J. | |
dc.contributor.author | Barnard, Marc R. | |
dc.contributor.author | Krueger, Lori A. | |
dc.contributor.author | Frelinger, Andrew L. III | |
dc.contributor.author | Michelson, Alan D. | |
dc.date | 2022-08-11T08:10:10.000 | |
dc.date.accessioned | 2022-08-23T16:58:23Z | |
dc.date.available | 2022-08-23T16:58:23Z | |
dc.date.issued | 2001-04-01 | |
dc.date.submitted | 2012-04-25 | |
dc.identifier.citation | Cytometry. 2001 Apr 1;43(4):308-13. | |
dc.identifier.issn | 0196-4763 (Linking) | |
dc.identifier.pmid | 11260598 | |
dc.identifier.uri | http://hdl.handle.net/20.500.14038/43355 | |
dc.description.abstract | BACKGROUND: Adherence of platelets to endothelial cells may be a significant event in the development of vascular thrombosis. Existing models, which examine platelet-endothelial cell interactions, compromise endothelial cell integrity or use radioactivity to identify platelets that adhere to endothelial cells. We report a novel method for in vitro detection of platelet-endothelial cell adhesion that allows endothelial cells to remain as an intact monolayer and for visualization of individual platelets. METHODS: Fluorescently labeled platelets were incubated with a confluent monolayer of endothelial cells. Laser scanning cytometry (LSC) identified platelets bound to endothelial cells based on their fluorescent signals. RESULTS: LSC detection of platelets reliably reproduced well-described findings of thrombin-induced platelet-endothelial cell adhesion. Results demonstrating reduced adhesion with a glycoprotein IIb-IIIa-specific blocking monoclonal antibody confirmed the specificity of the LSC detection of platelet-endothelial cell adhesion. CONCLUSIONS: LSC is a novel method for detecting platelet--endothelial cell adhesion. Its advantages over other methods are: (a) endothelial cells remain undisturbed and adherent throughout; (b) the ability to detect individual bound platelets and subpopulations; (c) the ability to store images and slides and then relocate, revisualize, and reanalyze individual cells or cell populations of interest; and (d) no radioactivity. | |
dc.language.iso | en_US | |
dc.relation | <a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11260598&dopt=Abstract">Link to article in PubMed</a> | |
dc.relation.url | http://dx.doi.org/10.1002/1097-0320(20010401)43:4<308::AID-CYTO1063>3.0.CO;2-9 | |
dc.subject | Hematology | |
dc.subject | Oncology | |
dc.subject | Pediatrics | |
dc.title | Laser scanning cytometry: a novel method for the detection of platelet--endothelial cell adhesion | |
dc.type | Journal Article | |
dc.source.journaltitle | Cytometry | |
dc.source.volume | 43 | |
dc.source.issue | 4 | |
dc.identifier.legacycoverpage | https://escholarship.umassmed.edu/peds_hematology/33 | |
dc.identifier.contextkey | 2796521 | |
html.description.abstract | <p>BACKGROUND: Adherence of platelets to endothelial cells may be a significant event in the development of vascular thrombosis. Existing models, which examine platelet-endothelial cell interactions, compromise endothelial cell integrity or use radioactivity to identify platelets that adhere to endothelial cells. We report a novel method for in vitro detection of platelet-endothelial cell adhesion that allows endothelial cells to remain as an intact monolayer and for visualization of individual platelets.</p> <p>METHODS: Fluorescently labeled platelets were incubated with a confluent monolayer of endothelial cells. Laser scanning cytometry (LSC) identified platelets bound to endothelial cells based on their fluorescent signals.</p> <p>RESULTS: LSC detection of platelets reliably reproduced well-described findings of thrombin-induced platelet-endothelial cell adhesion. Results demonstrating reduced adhesion with a glycoprotein IIb-IIIa-specific blocking monoclonal antibody confirmed the specificity of the LSC detection of platelet-endothelial cell adhesion.</p> <p>CONCLUSIONS: LSC is a novel method for detecting platelet--endothelial cell adhesion. Its advantages over other methods are: (a) endothelial cells remain undisturbed and adherent throughout; (b) the ability to detect individual bound platelets and subpopulations; (c) the ability to store images and slides and then relocate, revisualize, and reanalyze individual cells or cell populations of interest; and (d) no radioactivity.</p> | |
dc.identifier.submissionpath | peds_hematology/33 | |
dc.contributor.department | Department of Surgery | |
dc.contributor.department | Department of Pediatrics | |
dc.source.pages | 308-13 |