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dc.contributor.authorLian, Zheng
dc.contributor.authorWang, Le
dc.contributor.authorYamaga, Shigeru
dc.contributor.authorBonds, Wesley
dc.contributor.authorBeazer-Barclay, Y.
dc.contributor.authorKluger, Yuval
dc.contributor.authorGerstein, Mark B.
dc.contributor.authorNewburger, Peter E.
dc.contributor.authorBerliner, Nancy
dc.contributor.authorWeissman, Sherman M.
dc.date2022-08-11T08:10:10.000
dc.date.accessioned2022-08-23T16:58:23Z
dc.date.available2022-08-23T16:58:23Z
dc.date.issued2001-08-01
dc.date.submitted2012-04-25
dc.identifier.citationBlood. 2001 Aug 1;98(3):513-24. doi 10.1182/blood.V98.3.513
dc.identifier.issn0006-4971 (Linking)
dc.identifier.doi10.1182/blood.V98.3.513
dc.identifier.pmid11468144
dc.identifier.urihttp://hdl.handle.net/20.500.14038/43357
dc.description.abstractAlthough the mature neutrophil is one of the better characterized mammalian cell types, the mechanisms of myeloid differentiation are incompletely understood at the molecular level. A mouse promyelocytic cell line (MPRO), derived from murine bone marrow cells and arrested developmentally by a dominant-negative retinoic acid receptor, morphologically differentiates to mature neutrophils in the presence of 10 microM retinoic acid. An extensive catalog was prepared of the gene expression changes that occur during morphologic maturation. To do this, 3'-end differential display, oligonucleotide chip array hybridization, and 2-dimensional protein electrophoresis were used. A large number of genes whose mRNA levels are modulated during differentiation of MPRO cells were identified. The results suggest the involvement of several transcription regulatory factors not previously implicated in this process, but they also emphasize the importance of events other than the production of new transcription factors. Furthermore, gene expression patterns were compared at the level of mRNA and protein, and the correlation between 2 parameters was studied. (Blood. 2001;98:513-524)
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=11468144&dopt=Abstract">Link to article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1182/blood.V98.3.513
dc.subjectAnimals
dc.subjectCell Differentiation
dc.subjectCell Line
dc.subjectElectrophoresis, Gel, Two-Dimensional
dc.subjectGene Expression Regulation
dc.subject*Genomics
dc.subjectMass Spectrometry
dc.subjectMice
dc.subjectMyeloid Cells
dc.subjectNeutrophil Activation
dc.subjectOligonucleotide Array Sequence Analysis
dc.subjectProteins
dc.subjectProteome
dc.subjectRNA, Messenger
dc.subjectTransduction, Genetic
dc.subjectTretinoin
dc.subjectHematology
dc.subjectOncology
dc.subjectPediatrics
dc.titleGenomic and proteomic analysis of the myeloid differentiation program
dc.typeJournal Article
dc.source.journaltitleBlood
dc.source.volume98
dc.source.issue3
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/peds_hematology/35
dc.identifier.contextkey2796523
html.description.abstract<p>Although the mature neutrophil is one of the better characterized mammalian cell types, the mechanisms of myeloid differentiation are incompletely understood at the molecular level. A mouse promyelocytic cell line (MPRO), derived from murine bone marrow cells and arrested developmentally by a dominant-negative retinoic acid receptor, morphologically differentiates to mature neutrophils in the presence of 10 microM retinoic acid. An extensive catalog was prepared of the gene expression changes that occur during morphologic maturation. To do this, 3'-end differential display, oligonucleotide chip array hybridization, and 2-dimensional protein electrophoresis were used. A large number of genes whose mRNA levels are modulated during differentiation of MPRO cells were identified. The results suggest the involvement of several transcription regulatory factors not previously implicated in this process, but they also emphasize the importance of events other than the production of new transcription factors. Furthermore, gene expression patterns were compared at the level of mRNA and protein, and the correlation between 2 parameters was studied. (Blood. 2001;98:513-524)</p>
dc.identifier.submissionpathpeds_hematology/35
dc.contributor.departmentDepartment of Pediatrics
dc.source.pages513-24


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