Increased platelet reactivity and circulating monocyte-platelet aggregates in patients with stable coronary artery disease
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Authors
Furman, Mark I.Benoit, Stephen E.
Barnard, Marc R.
Valeri, C. Robert
Borbone, Marie L.
Becker, Richard C.
Hechtman, Herbert B.
Michelson, Alan D.
Document Type
Journal ArticlePublication Date
1998-02-01Keywords
Adenosine DiphosphateAdult
Angina Pectoris
Cell Adhesion
Cell Adhesion Molecules
Cell Count
Cell Degranulation
Cell Movement
Coronary Artery Disease
Coronary Disease
Coronary Vessels
Epinephrine
Female
Flow Cytometry
Humans
Leukocytes
Male
Middle Aged
Monocytes
P-Selectin
Peptide Fragments
Platelet Activation
Platelet Aggregation
Receptors, Thrombin
Thrombosis
Hematology
Oncology
Pediatrics
Metadata
Show full item recordAbstract
OBJECTIVES: We sought to examine whether patients with stable coronary artery disease (CAD) have increased platelet reactivity and an enhanced propensity to form monocyte-platelet aggregates. BACKGROUND: Platelet-dependent thrombosis and leukocyte infiltration into the vessel wall are characteristic cellular events seen in atherosclerosis. METHODS: Anticoagulated peripheral venous blood from 19 patients with stable CAD and 19 normal control subjects was incubated with or without various platelet agonists and analyzed by whole blood flow cytometry. RESULTS: Circulating degranulated platelets were increased in patients with CAD compared with control subjects (mean [+/- SEM] percent P-selectin-positive platelets: 2.1 +/- 0.2 vs. 1.5 +/- 0.2, p andlt; 0.01) and were more reactive to stimulation with 1 micromol/liter of adenosine diphosphate (ADP) (28.7 +/- 3.9 vs. 16.1 +/- 2.2, p andlt; 0.01), 1 micromol/liter of ADP/epinephrine (51.4 +/- 4.6 vs. 37.5 +/- 3.8, p andlt; 0.05) or 5 micromol/liter of thrombin receptor agonist peptide (TRAP) (65.7 +/- 6.8 vs. 20.2 +/- 5.1, p andlt; 0.01). Patients with stable CAD also had increased circulating monocyte-platelet aggregates compared with control subjects (percent platelet-positive monocytes: 15.3 +/- 3.0 vs. 6.3 +/- 0.9, p andlt; 0.01). Furthermore, patients with stable CAD formed more monocyte-platelet aggregates than did control subjects when their whole blood was stimulated with 1 micromol/liter of ADP (50.4 +/- 4.5 vs. 28.1 +/- 5.3, p andlt; 0.01), 1 micromol/liter of ADP/epinephrine (60.7 +/- 4.3 vs. 48.0 +/- 4.8, p andlt; 0.05) or 5 micromol/liter of TRAP (67.6 +/- 5.7 vs. 34.3 +/- 7.0, p andlt; 0.01). CONCLUSIONS: Patients with stable CAD have circulating activated platelets, circulating monocyte-platelet aggregates, increased platelet reactivity and an increased propensity to form monocyte-platelet aggregates.Source
J Am Coll Cardiol. 1998 Feb;31(2):352-8. doi 10.1016/S0735-1097(97)00510-XDOI
10.1016/S0735-1097(97)00510-XPermanent Link to this Item
http://hdl.handle.net/20.500.14038/43362PubMed ID
9462579Related Resources
Link to article in PubMedae974a485f413a2113503eed53cd6c53
10.1016/S0735-1097(97)00510-X