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dc.contributor.authorFurman, Mark I.
dc.contributor.authorBenoit, Stephen E.
dc.contributor.authorBarnard, Marc R.
dc.contributor.authorValeri, C. Robert
dc.contributor.authorBorbone, Marie L.
dc.contributor.authorBecker, Richard C.
dc.contributor.authorHechtman, Herbert B.
dc.contributor.authorMichelson, Alan D.
dc.date2022-08-11T08:10:10.000
dc.date.accessioned2022-08-23T16:58:25Z
dc.date.available2022-08-23T16:58:25Z
dc.date.issued1998-02-01
dc.date.submitted2012-04-25
dc.identifier.citationJ Am Coll Cardiol. 1998 Feb;31(2):352-8. doi 10.1016/S0735-1097(97)00510-X
dc.identifier.issn0735-1097 (Linking)
dc.identifier.doi10.1016/S0735-1097(97)00510-X
dc.identifier.pmid9462579
dc.identifier.urihttp://hdl.handle.net/20.500.14038/43362
dc.description.abstractOBJECTIVES: We sought to examine whether patients with stable coronary artery disease (CAD) have increased platelet reactivity and an enhanced propensity to form monocyte-platelet aggregates. BACKGROUND: Platelet-dependent thrombosis and leukocyte infiltration into the vessel wall are characteristic cellular events seen in atherosclerosis. METHODS: Anticoagulated peripheral venous blood from 19 patients with stable CAD and 19 normal control subjects was incubated with or without various platelet agonists and analyzed by whole blood flow cytometry. RESULTS: Circulating degranulated platelets were increased in patients with CAD compared with control subjects (mean [+/- SEM] percent P-selectin-positive platelets: 2.1 +/- 0.2 vs. 1.5 +/- 0.2, p andlt; 0.01) and were more reactive to stimulation with 1 micromol/liter of adenosine diphosphate (ADP) (28.7 +/- 3.9 vs. 16.1 +/- 2.2, p andlt; 0.01), 1 micromol/liter of ADP/epinephrine (51.4 +/- 4.6 vs. 37.5 +/- 3.8, p andlt; 0.05) or 5 micromol/liter of thrombin receptor agonist peptide (TRAP) (65.7 +/- 6.8 vs. 20.2 +/- 5.1, p andlt; 0.01). Patients with stable CAD also had increased circulating monocyte-platelet aggregates compared with control subjects (percent platelet-positive monocytes: 15.3 +/- 3.0 vs. 6.3 +/- 0.9, p andlt; 0.01). Furthermore, patients with stable CAD formed more monocyte-platelet aggregates than did control subjects when their whole blood was stimulated with 1 micromol/liter of ADP (50.4 +/- 4.5 vs. 28.1 +/- 5.3, p andlt; 0.01), 1 micromol/liter of ADP/epinephrine (60.7 +/- 4.3 vs. 48.0 +/- 4.8, p andlt; 0.05) or 5 micromol/liter of TRAP (67.6 +/- 5.7 vs. 34.3 +/- 7.0, p andlt; 0.01). CONCLUSIONS: Patients with stable CAD have circulating activated platelets, circulating monocyte-platelet aggregates, increased platelet reactivity and an increased propensity to form monocyte-platelet aggregates.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=9462579&dopt=Abstract">Link to article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1016/S0735-1097(97)00510-X
dc.subjectAdenosine Diphosphate
dc.subjectAdult
dc.subjectAngina Pectoris
dc.subjectCell Adhesion
dc.subjectCell Adhesion Molecules
dc.subjectCell Count
dc.subjectCell Degranulation
dc.subjectCell Movement
dc.subjectCoronary Artery Disease
dc.subjectCoronary Disease
dc.subjectCoronary Vessels
dc.subjectEpinephrine
dc.subjectFemale
dc.subjectFlow Cytometry
dc.subjectHumans
dc.subjectLeukocytes
dc.subjectMale
dc.subjectMiddle Aged
dc.subjectMonocytes
dc.subjectP-Selectin
dc.subjectPeptide Fragments
dc.subjectPlatelet Activation
dc.subjectPlatelet Aggregation
dc.subjectReceptors, Thrombin
dc.subjectThrombosis
dc.subjectHematology
dc.subjectOncology
dc.subjectPediatrics
dc.titleIncreased platelet reactivity and circulating monocyte-platelet aggregates in patients with stable coronary artery disease
dc.typeArticle
dc.source.journaltitleJournal of the American College of Cardiology
dc.source.volume31
dc.source.issue2
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/peds_hematology/4
dc.identifier.contextkey2796491
html.description.abstract<p>OBJECTIVES: We sought to examine whether patients with stable coronary artery disease (CAD) have increased platelet reactivity and an enhanced propensity to form monocyte-platelet aggregates.</p> <p>BACKGROUND: Platelet-dependent thrombosis and leukocyte infiltration into the vessel wall are characteristic cellular events seen in atherosclerosis.</p> <p>METHODS: Anticoagulated peripheral venous blood from 19 patients with stable CAD and 19 normal control subjects was incubated with or without various platelet agonists and analyzed by whole blood flow cytometry.</p> <p>RESULTS: Circulating degranulated platelets were increased in patients with CAD compared with control subjects (mean [+/- SEM] percent P-selectin-positive platelets: 2.1 +/- 0.2 vs. 1.5 +/- 0.2, p andlt; 0.01) and were more reactive to stimulation with 1 micromol/liter of adenosine diphosphate (ADP) (28.7 +/- 3.9 vs. 16.1 +/- 2.2, p andlt; 0.01), 1 micromol/liter of ADP/epinephrine (51.4 +/- 4.6 vs. 37.5 +/- 3.8, p andlt; 0.05) or 5 micromol/liter of thrombin receptor agonist peptide (TRAP) (65.7 +/- 6.8 vs. 20.2 +/- 5.1, p andlt; 0.01). Patients with stable CAD also had increased circulating monocyte-platelet aggregates compared with control subjects (percent platelet-positive monocytes: 15.3 +/- 3.0 vs. 6.3 +/- 0.9, p andlt; 0.01). Furthermore, patients with stable CAD formed more monocyte-platelet aggregates than did control subjects when their whole blood was stimulated with 1 micromol/liter of ADP (50.4 +/- 4.5 vs. 28.1 +/- 5.3, p andlt; 0.01), 1 micromol/liter of ADP/epinephrine (60.7 +/- 4.3 vs. 48.0 +/- 4.8, p andlt; 0.05) or 5 micromol/liter of TRAP (67.6 +/- 5.7 vs. 34.3 +/- 7.0, p andlt; 0.01).</p> <p>CONCLUSIONS: Patients with stable CAD have circulating activated platelets, circulating monocyte-platelet aggregates, increased platelet reactivity and an increased propensity to form monocyte-platelet aggregates.</p>
dc.identifier.submissionpathpeds_hematology/4
dc.contributor.departmentDepartment of Surgery
dc.contributor.departmentDepartment of Pediatrics
dc.contributor.departmentDepartment of Medicine
dc.source.pages352-8


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