The effect of IFN-gamma and TNF-alpha on the eosinophilic differentiation and NADPH oxidase activation of human HL-60 clone 15 cells
UMass Chan Affiliations
Department of PediatricsDocument Type
Journal ArticlePublication Date
2003-12-01Keywords
Biological MarkersButyric Acid
Carrier Proteins
Cell Differentiation
Cell Lineage
Clone Cells
Enzyme Activation
Eosinophils
Erythropoietin
Gene Expression Regulation
HL-60 Cells
Humans
Interferon-gamma
Interleukin-5
Maltose-Binding Proteins
Membrane Glycoproteins
NADPH Oxidase
Peroxidase
Tumor Necrosis Factor-alpha
Amino Acids, Peptides, and Proteins
Cell Biology
Hematology
Oncology
Pediatrics
Metadata
Show full item recordAbstract
The aim of this study was to investigate the effect of interferon-gamma (IFN-gamma) and tumor necrosis factor-alpha (TNF-alpha) on NADPH oxidase activity and gp91-phox gene expression in HL-60 clone 15 cells as they differentiate along the eosinophilic lineage. The results were compared to the eosoniphilic inducers interleukin-5 (IL-5) and butyric acid. IFN-gamma (100 U/ml) and TNF-alpha (1000 U/ml) or IL-5 (200 pM) caused a significant increase in the expression of the eosinophil peroxidase (EPO) and the major basic protein (MBP) genes. Similar results were observed when the cells were cultured with 0.5 mM butyric acid for 5 days. IFN-gamma (100 U/ml) and TNF-alpha (1000 U/ml) also caused a significant increase in superoxide release by HL-60 clone 15 cells after 2 days compared with control or with butyric acid-induced cells. After 5 days, these cytokines and butyric acid induced an even stronger release of superoxide. HL-60 clone 15 cells cultured with IFN-gamma and TNF-alpha for 2 days showed a significant increase in gp91-phox gene expression. We conclude that IFN-gamma and TNF-alpha are sufficient to induce the differentiation of HL-60 clone 15 cells to the eosinophilic lineage and to upregulate gp91-phox gene expression and activity of the NADPH oxidase system.Source
J Interferon Cytokine Res. 2003 Dec;23(12):737-44. doi 10.1089/107999003772084851. Link to article on publisher's website
DOI
10.1089/107999003772084851Permanent Link to this Item
http://hdl.handle.net/20.500.14038/43375PubMed ID
14769150Related Resources
Rights
This is a copy of an article published in the Journal of Interferon and Cytokine Research © 2003 copyright Mary Ann Liebert, Inc. and available online at: http://online.liebertpub.com.ae974a485f413a2113503eed53cd6c53
10.1089/107999003772084851