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dc.contributor.authorHimmelfarb, J.
dc.contributor.authorNelson, S.
dc.contributor.authorMcMonagle, E.
dc.contributor.authorHolbrook, D.
dc.contributor.authorBenoit, Stephen E.
dc.contributor.authorMichelson, Alan D.
dc.contributor.authorAult, Kenneth
dc.date2022-08-11T08:10:10.000
dc.date.accessioned2022-08-23T16:58:30Z
dc.date.available2022-08-23T16:58:30Z
dc.date.issued1998-07-01
dc.date.submitted2012-04-25
dc.identifier.citationAm J Kidney Dis. 1998 Jul;32(1):132-8. doi 10.1053/ajkd.1998.v32.pm9669434
dc.identifier.issn0272-6386 (Linking)
dc.identifier.doi10.1053/ajkd.1998.v32.pm9669434
dc.identifier.pmid9669434
dc.identifier.urihttp://hdl.handle.net/20.500.14038/43382
dc.description.abstractA bleeding diathesis caused by platelet dysfunction is a major cause of morbidity and mortality in patients with uremia. Platelet adhesion to vascular subendothelium is defective in uremia and depends on the interactions of the platelet glycoprotein (GP) Ib/IX complex with the vascular wall. We measured levels of platelet surface GPIb, platelet surface GPIX, plasma glycocalicin (a product of enzymatic cleavage of GPIb), and ristocetin-induced platelet agglutination (RIPA) in patients undergoing chronic hemodialysis compared with patients undergoing peritoneal dialysis and healthy controls. Patients undergoing chronic maintenance hemodialysis have higher levels of platelet surface expression of GPIb (187+/-10 fluorescent units; P andlt; 0.001) than either healthy controls (120+/-4 fluorescent units; P andlt; 0.001) or patients undergoing peritoneal dialysis (127+/-5 fluorescent units; P andlt; 0.001). Similar changes were observed in platelet surface GPIX. Plasma glycocalicin levels were elevated in chronic hemodialysis patients (71+/-5 nmol/L) compared with healthy controls (36+/-3 nmol/L; P andlt; 0.001). Plasma glycocalicin levels also increased progressively throughout the hemodialysis procedure. The slope of RIPA was significantly lower in chronic hemodialysis patients (46+/-3) than in either healthy controls (67+/-4; P andlt; 0.05) or peritoneal dialysis patients (62+/-2; P andlt; 0.05). In conclusion, patients undergoing chronic maintenance hemodialysis have increased plasma glycocalicin levels and decreased RIPA, which may contribute to diminished platelet adhesion to vascular subendothelium and increased bleeding associated with uremia.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=9669434&dopt=Abstract">Link to article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1053/ajkd.1998.v32.pm9669434
dc.subjectBlood Platelets
dc.subjectCase-Control Studies
dc.subjectHumans
dc.subjectPeritoneal Dialysis
dc.subjectPlatelet Aggregation
dc.subjectPlatelet Aggregation Inhibitors
dc.subjectPlatelet Glycoprotein GPIb-IX Complex
dc.subject*Renal Dialysis
dc.subjectRistocetin
dc.subjectUremia
dc.subjectHematology
dc.subjectOncology
dc.subjectPediatrics
dc.titleElevated plasma glycocalicin levels and decreased ristocetin-induced platelet agglutination in hemodialysis patients
dc.typeJournal Article
dc.source.journaltitleAmerican journal of kidney diseases : the official journal of the National Kidney Foundation
dc.source.volume32
dc.source.issue1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/peds_hematology/6
dc.identifier.contextkey2796493
html.description.abstract<p>A bleeding diathesis caused by platelet dysfunction is a major cause of morbidity and mortality in patients with uremia. Platelet adhesion to vascular subendothelium is defective in uremia and depends on the interactions of the platelet glycoprotein (GP) Ib/IX complex with the vascular wall. We measured levels of platelet surface GPIb, platelet surface GPIX, plasma glycocalicin (a product of enzymatic cleavage of GPIb), and ristocetin-induced platelet agglutination (RIPA) in patients undergoing chronic hemodialysis compared with patients undergoing peritoneal dialysis and healthy controls. Patients undergoing chronic maintenance hemodialysis have higher levels of platelet surface expression of GPIb (187+/-10 fluorescent units; P andlt; 0.001) than either healthy controls (120+/-4 fluorescent units; P andlt; 0.001) or patients undergoing peritoneal dialysis (127+/-5 fluorescent units; P andlt; 0.001). Similar changes were observed in platelet surface GPIX. Plasma glycocalicin levels were elevated in chronic hemodialysis patients (71+/-5 nmol/L) compared with healthy controls (36+/-3 nmol/L; P andlt; 0.001). Plasma glycocalicin levels also increased progressively throughout the hemodialysis procedure. The slope of RIPA was significantly lower in chronic hemodialysis patients (46+/-3) than in either healthy controls (67+/-4; P andlt; 0.05) or peritoneal dialysis patients (62+/-2; P andlt; 0.05). In conclusion, patients undergoing chronic maintenance hemodialysis have increased plasma glycocalicin levels and decreased RIPA, which may contribute to diminished platelet adhesion to vascular subendothelium and increased bleeding associated with uremia.</p>
dc.identifier.submissionpathpeds_hematology/6
dc.contributor.departmentDepartment of Pediatrics
dc.source.pages132-8


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