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dc.contributor.authorZhang, Xueqing
dc.contributor.authorKluger, Yuval
dc.contributor.authorNakayama, Yasuhiro
dc.contributor.authorPoddar, Ranjana
dc.contributor.authorWhitney, Constance
dc.contributor.authorDeTora, Adam
dc.contributor.authorWeissman, Sherman M.
dc.contributor.authorNewburger, Peter E.
dc.date2022-08-11T08:10:10.000
dc.date.accessioned2022-08-23T16:58:31Z
dc.date.available2022-08-23T16:58:31Z
dc.date.issued2004-02-01
dc.date.submitted2012-04-25
dc.identifier.citationJ Leukoc Biol. 2004 Feb;75(2):358-72. Epub 2003 Nov 21. doi: 10.1189/jlb.0903412
dc.identifier.issn0741-5400 (Linking)
dc.identifier.doi10.1189/jlb.0903412
dc.identifier.pmid14634056
dc.identifier.urihttp://hdl.handle.net/20.500.14038/43385
dc.description.abstractNeutrophils provide an essential defense against bacterial and fungal infection and play a major role in tissue damage during inflammation. Using oligonucleotide microarrays, we have examined the time course of changes in gene expression induced by stimulation with live, opsonized Escherichia coli, soluble lipopolysaccharide, and the chemoattractant formyl-methionyl-leucyl-phenylalanine. The results indicate that activated neutrophils generate a broad and vigorous set of alterations in gene expression. The responses included changes in the levels of transcripts encoding 148 transcription factors and chromatin-remodeling genes and 95 regulators of protein synthesis or stability. Clustering analysis showed distinct temporal patterns with many rapid changes in gene expression within the first hour of exposure. In addition to the temporal clustering of genes, we also observed rather different profiles associated with each stimulus, suggesting that even a nonvirulent organism such as E. coli is able to play a dynamic role in shaping the inflammatory response. Principal component analysis of transcription factor genes demonstrated clear separation of the neutrophil-response clusters from those of resting and stimulated human monocytes. The present study indicates that combinatorial transcriptional regulation including alterations of chromatin structure may play a role in the rapid changes in gene expression that occur in these terminally differentiated cells.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=14634056&dopt=Abstract">Link to article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1189/jlb.0903412
dc.subjectChromatin Assembly and Disassembly
dc.subjectCluster Analysis
dc.subjectEscherichia coli
dc.subjectGene Expression Profiling
dc.subjectGene Expression Regulation
dc.subjectHumans
dc.subjectInflammation Mediators
dc.subjectLipopolysaccharides
dc.subjectN-Formylmethionine Leucyl-Phenylalanine
dc.subjectNeutrophils
dc.subjectTime Factors
dc.subjectTranscription Factors
dc.subjectHematology
dc.subjectOncology
dc.subjectPediatrics
dc.titleGene expression in mature neutrophils: early responses to inflammatory stimuli
dc.typeJournal Article
dc.source.journaltitleJournal of leukocyte biology
dc.source.volume75
dc.source.issue2
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/peds_hematology/62
dc.identifier.contextkey2796552
html.description.abstract<p>Neutrophils provide an essential defense against bacterial and fungal infection and play a major role in tissue damage during inflammation. Using oligonucleotide microarrays, we have examined the time course of changes in gene expression induced by stimulation with live, opsonized Escherichia coli, soluble lipopolysaccharide, and the chemoattractant formyl-methionyl-leucyl-phenylalanine. The results indicate that activated neutrophils generate a broad and vigorous set of alterations in gene expression. The responses included changes in the levels of transcripts encoding 148 transcription factors and chromatin-remodeling genes and 95 regulators of protein synthesis or stability. Clustering analysis showed distinct temporal patterns with many rapid changes in gene expression within the first hour of exposure. In addition to the temporal clustering of genes, we also observed rather different profiles associated with each stimulus, suggesting that even a nonvirulent organism such as E. coli is able to play a dynamic role in shaping the inflammatory response. Principal component analysis of transcription factor genes demonstrated clear separation of the neutrophil-response clusters from those of resting and stimulated human monocytes. The present study indicates that combinatorial transcriptional regulation including alterations of chromatin structure may play a role in the rapid changes in gene expression that occur in these terminally differentiated cells.</p>
dc.identifier.submissionpathpeds_hematology/62
dc.contributor.departmentDepartment of Pediatrics
dc.source.pages358-72


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