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dc.contributor.authorCondino-Neto, Antonio
dc.contributor.authorWhitney, Constance
dc.contributor.authorNewburger, Peter E.
dc.date2022-08-11T08:10:11.000
dc.date.accessioned2022-08-23T16:58:38Z
dc.date.available2022-08-23T16:58:38Z
dc.date.issued1998-11-01
dc.date.submitted2012-04-25
dc.identifier.citationJ Immunol. 1998 Nov 1;161(9):4960-7.
dc.identifier.issn0022-1767 (Linking)
dc.identifier.pmid9794432
dc.identifier.urihttp://hdl.handle.net/20.500.14038/43412
dc.description.abstractWe investigated the effects of dexamethasone or indomethacin on the NADPH oxidase activity, cytochrome b558 content, and expression of genes encoding the components gp91-phox and p47-phox of the NADPH oxidase system in the human monocytic THP-1 cell line, differentiated with IFN-gamma and TNF-alpha, alone or in combination, for up to 7 days. IFN-gamma and TNF-alpha, alone or in combination, caused a significant up-regulation of the NADPH oxidase system as reflected by an enhancement of the PMA-stimulated superoxide release, cytochrome b558 content, and expression of gp91-phox and p47-phox genes on both days 2 and 7 of cell culture. Noteworthy was the tremendous synergism between IFN-gamma and TNF-alpha for all studied parameters. Dexamethasone down-regulated the NADPH oxidase system of cytokine-differentiated THP-1 cells as assessed by an inhibition on the PMA-stimulated superoxide release, cytochrome b558 content, and expression of the gp91-phox and p47-phox genes. The nuclear run-on assays indicated that dexamethasone down-regulated the NADPH oxidase system at least in part by inhibiting the transcription of gp91-phox and p47-phox genes. Indomethacin inhibited only the PMA-stimulated superoxide release of THP-1 cells differentiated with IFN-gamma and TNF-alpha during 7 days. None of the other parameters was affected by indomethacin. We conclude that dexamethasone down-regulates the NADPH oxidase system at least in part by inhibiting the expression of genes encoding the gp91-phox and p47-phox components of the NADPH oxidase system.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=9794432&dopt=Abstract">Link to article in PubMed</a>
dc.relation.urlhttp://www.jimmunol.org/content/161/9/4960.full.pdf+html
dc.subjectAnti-Inflammatory Agents
dc.subjectAnti-Inflammatory Agents, Non-Steroidal
dc.subjectCells, Cultured
dc.subjectCytochrome b Group
dc.subjectDexamethasone
dc.subjectEnzyme Inhibitors
dc.subjectGene Expression Regulation, Enzymologic
dc.subjectHumans
dc.subjectIndomethacin
dc.subjectInterferon-gamma
dc.subjectLeukemia, Monocytic, Acute
dc.subjectMembrane Glycoproteins
dc.subjectMonocytes
dc.subjectNADPH Oxidase
dc.subjectNeoplasm Proteins
dc.subjectPhosphoproteins
dc.subjectSuperoxides
dc.subjectTetradecanoylphorbol Acetate
dc.subjectTumor Cells, Cultured
dc.subjectTumor Necrosis Factor-alpha
dc.subjectHematology
dc.subjectOncology
dc.subjectPediatrics
dc.titleDexamethasone but not indomethacin inhibits human phagocyte nicotinamide adenine dinucleotide phosphate oxidase activity by down-regulating expression of genes encoding oxidase components
dc.typeArticle
dc.source.journaltitleJournal of immunology (Baltimore, Md. : 1950)
dc.source.volume161
dc.source.issue9
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/peds_hematology/9
dc.identifier.contextkey2796496
html.description.abstract<p>We investigated the effects of dexamethasone or indomethacin on the NADPH oxidase activity, cytochrome b558 content, and expression of genes encoding the components gp91-phox and p47-phox of the NADPH oxidase system in the human monocytic THP-1 cell line, differentiated with IFN-gamma and TNF-alpha, alone or in combination, for up to 7 days. IFN-gamma and TNF-alpha, alone or in combination, caused a significant up-regulation of the NADPH oxidase system as reflected by an enhancement of the PMA-stimulated superoxide release, cytochrome b558 content, and expression of gp91-phox and p47-phox genes on both days 2 and 7 of cell culture. Noteworthy was the tremendous synergism between IFN-gamma and TNF-alpha for all studied parameters. Dexamethasone down-regulated the NADPH oxidase system of cytokine-differentiated THP-1 cells as assessed by an inhibition on the PMA-stimulated superoxide release, cytochrome b558 content, and expression of the gp91-phox and p47-phox genes. The nuclear run-on assays indicated that dexamethasone down-regulated the NADPH oxidase system at least in part by inhibiting the transcription of gp91-phox and p47-phox genes. Indomethacin inhibited only the PMA-stimulated superoxide release of THP-1 cells differentiated with IFN-gamma and TNF-alpha during 7 days. None of the other parameters was affected by indomethacin. We conclude that dexamethasone down-regulates the NADPH oxidase system at least in part by inhibiting the expression of genes encoding the gp91-phox and p47-phox components of the NADPH oxidase system.</p>
dc.identifier.submissionpathpeds_hematology/9
dc.contributor.departmentDepartment of Pediatrics
dc.source.pages4960-7


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