CD4 down-modulation by human immunodeficiency virus type 1 Nef correlates with the efficiency of viral replication and with CD4(+) T-cell depletion in human lymphoid tissue ex vivo
Greenough, Thomas C.
Sullivan, John L.
Document TypeJournal Article
*CD4 Lymphocyte Count
Gene Products, nef
nef Gene Products, Human Immunodeficiency Virus
Immunology and Infectious Disease
MetadataShow full item record
AbstractThe human immunodeficiency virus type 1 (HIV-1) Nef protein is an important virulence factor. Nef has several functions, including down-modulation of CD4 and class I major histocompatibility complex cell surface expression, enhancement of virion infectivity, and stimulation of viral replication in peripheral blood mononuclear cells. Nef also increases HIV-1 replication in human lymphoid tissue (HLT) ex vivo. We analyzed recombinant and primary nef alleles with highly divergent activity in different in vitro assays to clarify which of these Nef activities are functionally linked. Our results demonstrate that Nef activity in CD4 down-regulation correlates significantly with the efficiency of HIV-1 replication and with the severity of CD4(+) T-cell depletion in HLT. In conclusion, HIV-1 Nef variants with increased activity in CD4 down-modulation would cause severe depletion of CD4(+) T cells in lymphoid tissues and accelerate AIDS progression.
SourceJ Virol. 2001 Nov;75(21):10113-7. Link to article on publisher's site
Permanent Link to this Itemhttp://hdl.handle.net/20.500.14038/43437
Related ResourcesLink to Article in PubMed