Cytomegalovirus (CMV) IE1- and pp65-specific CD8+ T cell responses broaden over time after primary CMV infection in infants
Authors
Gibson, Laura L.Dooley, Sheryl
Trzmielina, Sonia
Somasundaran, Mohan
Fisher, Donna
Revello, Maria Grazia
Luzuriaga, Katherine
Document Type
Journal ArticlePublication Date
2007-06-15Keywords
AllelesAmino Acid Sequence
Antigens, Viral
CD8-Positive T-Lymphocytes
Cell Line
Cohort Studies
Cytomegalovirus
Cytomegalovirus Infections
Humans
Immediate-Early Proteins
Infant
Leukocytes, Mononuclear
Peptide Mapping
Phosphoproteins
Time Factors
Viral Load
Viral Matrix Proteins
Viral Proteins
Immunology and Infectious Disease
Pediatrics
Metadata
Show full item recordAbstract
Cytomegalovirus (CMV) infection remains a significant cause of morbidity and mortality in young children. We have previously shown that CD8+ T cell responses to CMV pp65 or IE1 protein were readily detectable in children with congenital or postnatal CMV infection. Here, we have further characterized the evolution of the peptide specificity of these responses in 7 infants(median, 5 peptides/infant) were targeted, and most (61%) represented sequences not previously reported. Peptide specificity remained stable or broadened over time despite the clearance of CMV viremia. Loss of peptide recognition was not observed. Responses with the highest functional peptide avidity were not necessarily detected earliest. These data provide additional evidence that young infants can generate diverse CMV-specific CD8+ T cell responses but show that early responses may exhibit relatively focused peptide specificity and lower peptide avidity.Source
J Infect Dis. 2007 Jun 15;195(12):1789-98. Epub 2007 May 8. Link to article on publisher's siteDOI
10.1086/518042Permanent Link to this Item
http://hdl.handle.net/20.500.14038/43475PubMed ID
17492595Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1086/518042