Lack of alternative coreceptor use by pediatric HIV-1 R5 isolates for infection of primary cord or adult peripheral blood mononuclear cells
AuthorsSullivan, W. Matthew
Hudson, Richard W. Jr.
Leif, Jean H.
Clapham, Paul R.
UMass Chan AffiliationsDepartment of Molecular Genetics and Microbiology
Department of Medicine, Division of Diabetes
Program in Molecular Medicine
Department of Pediatrics
HIV Reverse Transcriptase
Immunology and Infectious Disease
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AbstractHIV-1 infection of neonates results in an extended acute period of virus replication, frequent neurological problems and reduced survival compared to adults. In adults, R5 viruses mainly infect CCR5(+) CD4(+) memory T-cells. In neonates, CCR5(+) memory T-cells form a substantially smaller fraction of total lymphocytes. We therefore tested whether alternative coreceptors confer infection of lymphocytes by pediatric isolates. Pediatric HIV-1 R5 isolates failed to replicate in Delta32/Delta32 CCR5 PBMCs or in cord PBMCs treated with a CCR5 inhibitor. These results do not indicate a role for alternative coreceptors and provide support for CCR5 inhibitors in the therapy of HIV-1(+) neonates.
SourceArch Virol. 2008;153(2):363-6. Epub 2007 Dec 13. Link to article on publisher's site
Permanent Link to this Itemhttp://hdl.handle.net/20.500.14038/43478
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