Quantification of Z-AAT by a Z-Specific "Sandwich" ELISA
| dc.contributor.author | Borel, Florie | |
| dc.contributor.author | Tang, Qiushi | |
| dc.contributor.author | Mueller, Christian | |
| dc.date | 2022-08-11T08:10:13.000 | |
| dc.date.accessioned | 2022-08-23T16:59:27Z | |
| dc.date.available | 2022-08-23T16:59:27Z | |
| dc.date.issued | 2017-07-28 | |
| dc.date.submitted | 2017-08-28 | |
| dc.identifier.citation | Methods Mol Biol. 2017;1639:223-226. doi: 10.1007/978-1-4939-7163-3_22. <a href="https://doi.org/10.1007/978-1-4939-7163-3_22">Link to article on publisher's site</a> | |
| dc.identifier.issn | 1064-3745 (Linking) | |
| dc.identifier.doi | 10.1007/978-1-4939-7163-3_22 | |
| dc.identifier.pmid | 28752462 | |
| dc.identifier.uri | http://hdl.handle.net/20.500.14038/43590 | |
| dc.description.abstract | This protocol describes an enzyme-linked immunosorbent assay (ELISA) to specifically detect Z-alpha-1 antitrypsin (AAT), the most common protein variant associated with alpha-1 antitrypsin deficiency. This "sandwich" ELISA relies on an anti-Z-AAT specific capture antibody and a HRP-conjugated anti-AAT detection antibody. This method would be of interest to identify and quantify Z-AAT in a variety of samples such as cell culture medium, cell or tissue lysate, animal or patient serum. Because this method is specific and sensitive, it would be particularly valuable for detection of Z-AAT in the presence of background M-AAT, for instance when quantifying silencing of Z-AAT in patients undergoing M-AAT augmentation therapy. | |
| dc.language.iso | en_US | |
| dc.relation | <a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=28752462&dopt=Abstract">Link to Article in PubMed</a> | |
| dc.relation.url | https://doi.org/10.1007/978-1-4939-7163-3_22 | |
| dc.subject | AAT | |
| dc.subject | Alpha-1 antitrypsin | |
| dc.subject | Enzyme-linked immunosorbent assay | |
| dc.subject | Sandwich ELISA | |
| dc.subject | UMMS-PiZ-mAb | |
| dc.subject | Z-AAT | |
| dc.subject | Molecular Biology | |
| dc.subject | Research Methods in Life Sciences | |
| dc.title | Quantification of Z-AAT by a Z-Specific "Sandwich" ELISA | |
| dc.type | Book Chapter | |
| dc.source.booktitle | Methods in molecular biology (Clifton, N.J.) | |
| dc.source.volume | 1639 | |
| dc.identifier.legacycoverpage | https://escholarship.umassmed.edu/peds_pp/160 | |
| dc.identifier.contextkey | 10667371 | |
| html.description.abstract | <p>This protocol describes an enzyme-linked immunosorbent assay (ELISA) to specifically detect Z-alpha-1 antitrypsin (AAT), the most common protein variant associated with alpha-1 antitrypsin deficiency. This "sandwich" ELISA relies on an anti-Z-AAT specific capture antibody and a HRP-conjugated anti-AAT detection antibody. This method would be of interest to identify and quantify Z-AAT in a variety of samples such as cell culture medium, cell or tissue lysate, animal or patient serum. Because this method is specific and sensitive, it would be particularly valuable for detection of Z-AAT in the presence of background M-AAT, for instance when quantifying silencing of Z-AAT in patients undergoing M-AAT augmentation therapy.</p> | |
| dc.identifier.submissionpath | peds_pp/160 | |
| dc.contributor.department | Department of Pediatrics, Division of Pulmonary and Allergy | |
| dc.contributor.department | Horae Gene Therapy Center | |
| dc.source.pages | 223-226 |