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    Quantification of Murine AAT by Direct ELISA

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    Authors
    Cox, Andrew
    Mueller, Christian
    UMass Chan Affiliations
    Department of Pediatrics, Division of Pulmonary and Allergy
    Horae Gene Therapy Center
    Document Type
    Book Chapter
    Publication Date
    2017-07-28
    Keywords
    AAT
    Alpha-1 antitrypsin
    Direct ELISA
    Enzyme-linked immunosorbent assay
    Murine AAT
    Molecular Biology
    Research Methods in Life Sciences
    
    Metadata
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    Link to Full Text
    https://doi.org/10.1007/978-1-4939-7163-3_21
    Abstract
    This methods chapter elaborates on how a direct enzyme-linked immunosorbent assay (ELISA) is used to specifically detect and quantify murine alpha-1 antitrypsin (AAT). As a direct ELISA, it lacks some sensitivity as compared to the "sandwich" ELISA method; however, it does reliably differentiate between samples with varying amounts of the mouse AAT protein. This protocol relies on the principle of adsorption to coat each well with sera proteins, whereas detection occurs specifically using a two-step antibody combination. This procedure effectively identifies and quantifies murine AAT from a wide variety of samples including mouse serum, cell culture medium, and cell or tissue lysate.
    Source
    Methods Mol Biol. 2017;1639:217-222. doi: 10.1007/978-1-4939-7163-3_21. Link to article on publisher's site
    DOI
    10.1007/978-1-4939-7163-3_21
    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/43591
    PubMed ID
    28752461
    Related Resources
    Link to Article in PubMed
    ae974a485f413a2113503eed53cd6c53
    10.1007/978-1-4939-7163-3_21
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