Show simple item record

dc.contributor.authorCox, Andrew
dc.contributor.authorMueller, Christian
dc.date2022-08-11T08:10:13.000
dc.date.accessioned2022-08-23T16:59:28Z
dc.date.available2022-08-23T16:59:28Z
dc.date.issued2017-07-28
dc.date.submitted2017-08-28
dc.identifier.citationMethods Mol Biol. 2017;1639:217-222. doi: 10.1007/978-1-4939-7163-3_21. <a href="https://doi.org/10.1007/978-1-4939-7163-3_21">Link to article on publisher's site</a>
dc.identifier.issn1064-3745 (Linking)
dc.identifier.doi10.1007/978-1-4939-7163-3_21
dc.identifier.pmid28752461
dc.identifier.urihttp://hdl.handle.net/20.500.14038/43591
dc.description.abstractThis methods chapter elaborates on how a direct enzyme-linked immunosorbent assay (ELISA) is used to specifically detect and quantify murine alpha-1 antitrypsin (AAT). As a direct ELISA, it lacks some sensitivity as compared to the "sandwich" ELISA method; however, it does reliably differentiate between samples with varying amounts of the mouse AAT protein. This protocol relies on the principle of adsorption to coat each well with sera proteins, whereas detection occurs specifically using a two-step antibody combination. This procedure effectively identifies and quantifies murine AAT from a wide variety of samples including mouse serum, cell culture medium, and cell or tissue lysate.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=28752461&dopt=Abstract">Link to Article in PubMed</a>
dc.relation.urlhttps://doi.org/10.1007/978-1-4939-7163-3_21
dc.subjectAAT
dc.subjectAlpha-1 antitrypsin
dc.subjectDirect ELISA
dc.subjectEnzyme-linked immunosorbent assay
dc.subjectMurine AAT
dc.subjectMolecular Biology
dc.subjectResearch Methods in Life Sciences
dc.titleQuantification of Murine AAT by Direct ELISA
dc.typeBook Chapter
dc.source.booktitleMethods in molecular biology (Clifton, N.J.)
dc.source.volume1639
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/peds_pp/161
dc.identifier.contextkey10667372
html.description.abstract<p>This methods chapter elaborates on how a direct enzyme-linked immunosorbent assay (ELISA) is used to specifically detect and quantify murine alpha-1 antitrypsin (AAT). As a direct ELISA, it lacks some sensitivity as compared to the "sandwich" ELISA method; however, it does reliably differentiate between samples with varying amounts of the mouse AAT protein. This protocol relies on the principle of adsorption to coat each well with sera proteins, whereas detection occurs specifically using a two-step antibody combination. This procedure effectively identifies and quantifies murine AAT from a wide variety of samples including mouse serum, cell culture medium, and cell or tissue lysate.</p>
dc.identifier.submissionpathpeds_pp/161
dc.contributor.departmentDepartment of Pediatrics, Division of Pulmonary and Allergy
dc.contributor.departmentHorae Gene Therapy Center
dc.source.pages217-222


This item appears in the following Collection(s)

Show simple item record