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dc.contributor.authorTang, Qiushi
dc.contributor.authorGruntman, Alisha
dc.contributor.authorFlotte, Terence R.
dc.date2022-08-11T08:10:13.000
dc.date.accessioned2022-08-23T16:59:28Z
dc.date.available2022-08-23T16:59:28Z
dc.date.issued2017-07-28
dc.date.submitted2017-08-28
dc.identifier.citationMethods Mol Biol. 2017;1639:211-216. doi: 10.1007/978-1-4939-7163-3_20. <a href="https://doi.org/10.1007/978-1-4939-7163-3_20">Link to article on publisher's site</a>
dc.identifier.issn1064-3745 (Linking)
dc.identifier.doi10.1007/978-1-4939-7163-3_20
dc.identifier.pmid28752460
dc.identifier.urihttp://hdl.handle.net/20.500.14038/43592
dc.description.abstractIn this chapter we describe an enzyme-linked immunosorbent assay (ELISA) to quantitatively measure human alpha-1 antitrypsin (AAT) protein levels in serum, other body fluids or liquid media. This assay can be used to measure the expression of the human AAT (hAAT) gene in a variety of gene transfer or gene downregulation experiments.A hAAT-specific capture antibody and a HRP-conjugated anti-AAT detection antibody are used in this assay. The conjugated anti-AAT used in this protocol, instead of the typical sandwich which employs an unconjugated antibody followed by a specifically conjugated IgG, makes the assay simpler and decreases variability. This provides a useful tool to evaluate the AAT levels in clinical and research samples and can allow fairly rapid testing of a large number of samples.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=28752460&dopt=Abstract">Link to Article in PubMed</a>
dc.relation.urlhttps://doi.org/10.1007/978-1-4939-7163-3_20
dc.subjectAAT
dc.subjectAlpha-1 antitrypsin
dc.subjectEnzyme-linked immunosorbent assay
dc.subjectSandwich ELISA
dc.subjectMolecular Biology
dc.subjectResearch Methods in Life Sciences
dc.titleQuantification of Total Human Alpha-1 Antitrypsin by Sandwich ELISA
dc.typeBook Chapter
dc.source.booktitleMethods in molecular biology (Clifton, N.J.)
dc.source.volume1639
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/peds_pp/162
dc.identifier.contextkey10667373
html.description.abstract<p>In this chapter we describe an enzyme-linked immunosorbent assay (ELISA) to quantitatively measure human alpha-1 antitrypsin (AAT) protein levels in serum, other body fluids or liquid media. This assay can be used to measure the expression of the human AAT (hAAT) gene in a variety of gene transfer or gene downregulation experiments.A hAAT-specific capture antibody and a HRP-conjugated anti-AAT detection antibody are used in this assay. The conjugated anti-AAT used in this protocol, instead of the typical sandwich which employs an unconjugated antibody followed by a specifically conjugated IgG, makes the assay simpler and decreases variability. This provides a useful tool to evaluate the AAT levels in clinical and research samples and can allow fairly rapid testing of a large number of samples.</p>
dc.identifier.submissionpathpeds_pp/162
dc.contributor.departmentDepartment of Pediatrics, Division of Pulmonary and Allergy
dc.contributor.departmentHorae Gene Therapy Center
dc.source.pages211-216


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