Quantification of Total Human Alpha-1 Antitrypsin by Sandwich ELISA
dc.contributor.author | Tang, Qiushi | |
dc.contributor.author | Gruntman, Alisha | |
dc.contributor.author | Flotte, Terence R. | |
dc.date | 2022-08-11T08:10:13.000 | |
dc.date.accessioned | 2022-08-23T16:59:28Z | |
dc.date.available | 2022-08-23T16:59:28Z | |
dc.date.issued | 2017-07-28 | |
dc.date.submitted | 2017-08-28 | |
dc.identifier.citation | Methods Mol Biol. 2017;1639:211-216. doi: 10.1007/978-1-4939-7163-3_20. <a href="https://doi.org/10.1007/978-1-4939-7163-3_20">Link to article on publisher's site</a> | |
dc.identifier.issn | 1064-3745 (Linking) | |
dc.identifier.doi | 10.1007/978-1-4939-7163-3_20 | |
dc.identifier.pmid | 28752460 | |
dc.identifier.uri | http://hdl.handle.net/20.500.14038/43592 | |
dc.description.abstract | In this chapter we describe an enzyme-linked immunosorbent assay (ELISA) to quantitatively measure human alpha-1 antitrypsin (AAT) protein levels in serum, other body fluids or liquid media. This assay can be used to measure the expression of the human AAT (hAAT) gene in a variety of gene transfer or gene downregulation experiments.A hAAT-specific capture antibody and a HRP-conjugated anti-AAT detection antibody are used in this assay. The conjugated anti-AAT used in this protocol, instead of the typical sandwich which employs an unconjugated antibody followed by a specifically conjugated IgG, makes the assay simpler and decreases variability. This provides a useful tool to evaluate the AAT levels in clinical and research samples and can allow fairly rapid testing of a large number of samples. | |
dc.language.iso | en_US | |
dc.relation | <a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=28752460&dopt=Abstract">Link to Article in PubMed</a> | |
dc.relation.url | https://doi.org/10.1007/978-1-4939-7163-3_20 | |
dc.subject | AAT | |
dc.subject | Alpha-1 antitrypsin | |
dc.subject | Enzyme-linked immunosorbent assay | |
dc.subject | Sandwich ELISA | |
dc.subject | Molecular Biology | |
dc.subject | Research Methods in Life Sciences | |
dc.title | Quantification of Total Human Alpha-1 Antitrypsin by Sandwich ELISA | |
dc.type | Book Chapter | |
dc.source.booktitle | Methods in molecular biology (Clifton, N.J.) | |
dc.source.volume | 1639 | |
dc.identifier.legacycoverpage | https://escholarship.umassmed.edu/peds_pp/162 | |
dc.identifier.contextkey | 10667373 | |
html.description.abstract | <p>In this chapter we describe an enzyme-linked immunosorbent assay (ELISA) to quantitatively measure human alpha-1 antitrypsin (AAT) protein levels in serum, other body fluids or liquid media. This assay can be used to measure the expression of the human AAT (hAAT) gene in a variety of gene transfer or gene downregulation experiments.A hAAT-specific capture antibody and a HRP-conjugated anti-AAT detection antibody are used in this assay. The conjugated anti-AAT used in this protocol, instead of the typical sandwich which employs an unconjugated antibody followed by a specifically conjugated IgG, makes the assay simpler and decreases variability. This provides a useful tool to evaluate the AAT levels in clinical and research samples and can allow fairly rapid testing of a large number of samples.</p> | |
dc.identifier.submissionpath | peds_pp/162 | |
dc.contributor.department | Department of Pediatrics, Division of Pulmonary and Allergy | |
dc.contributor.department | Horae Gene Therapy Center | |
dc.source.pages | 211-216 |