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    Evidence of insulin-like growth factor binding protein-3 proteolysis during growth hormone stimulation testing

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    Authors
    Nwosu, Benjamin U.
    Soyka, Leslie A.
    Angelescu, Amanda
    Lee, Mary M.
    UMass Chan Affiliations
    Department of Cell Biology
    Department of Pediatrics, Division of Endocrinology
    Document Type
    Journal Article
    Publication Date
    2011-04-14
    Keywords
    Arginine
    Child
    Dwarfism
    Female
    Growth Hormone-Releasing Hormone
    Human Growth Hormone
    Humans
    Insulin-Like Growth Factor Binding Protein 3
    Male
    Prospective Studies
    Puberty
    Endocrinology, Diabetes, and Metabolism
    Pediatrics
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    Link to Full Text
    http://dx.doi.org/10.1515/JPEM.2011.088
    Abstract
    OBJECTIVES: The ternary complex is composed of insulin-like growth factor (IGF)-I, IGF binding protein (IGFBP)-3 and acid labile subunit (ALS). Growth hormone (GH) promotes IGFBP-3 proteolysis to release free IGF-I, ALS, and IGFBP-3 fragments. Our aim was to determine whether elevated GH levels during GH stimulation testing would trigger IGFBP-3 proteolysis. DESIGN: This prospective study of 10 short prepubertal children (height standard deviation score -2.37 +/- 0.31) used arginine and GH releasing hormone stimulation to study dynamic changes in the ternary complex moieties. IGFBP-3 was measured in two assays: a radioimmunoassay (RIA) that detects both cleaved and intact IGFBP-3; and an immunochemiluminescence assay (ICMA) that detects only intact IGFBP-3. RESULTS: IGFBP-3 measured by RIA increased by 19% (p < 0.05), while IGFBP-3 measured by ICMA did not significantly increase (6.1%). CONCLUSION: The significant increase in IGFBP-3 measured by RIA, but not ICMA, provides evidence of IGFBP-3 proteolysis during acute GH stimulation.
    Source
    J Pediatr Endocrinol Metab. 2011;24(3-4):163-7. Link to article on publisher's website
    DOI
    10.1515/JPEM.2011.088
    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/43757
    PubMed ID
    21648284
    Related Resources
    Link to Article in PubMed
    ae974a485f413a2113503eed53cd6c53
    10.1515/JPEM.2011.088
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