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dc.contributor.authorChen, B.
dc.contributor.authorKapturczak, M. H.
dc.contributor.authorJoseph, R.
dc.contributor.authorGeorge, J. F.
dc.contributor.authorCampbell-Thompson, M.
dc.contributor.authorWasserfall, Clive H.
dc.contributor.authorAtkinson, Mark A.
dc.contributor.authorTisher, C. C.
dc.contributor.authorFlotte, Terence R.
dc.contributor.authorArgarwal, A.
dc.contributor.authorChen, S.
dc.date2022-08-11T08:10:14.000
dc.date.accessioned2022-08-23T17:00:28Z
dc.date.available2022-08-23T17:00:28Z
dc.date.issued2007-05-26
dc.date.submitted2012-01-11
dc.identifier.citationAm J Transplant. 2007 May;7(5):1112-20. <a href="http://dx.doi.org/10.1111/j.1600-6143.2007.01772.x">Link to article on publisher's site</a>
dc.identifier.issn1600-6135 (Linking)
dc.identifier.doi10.1111/j.1600-6143.2007.01772.x
dc.identifier.pmid17456199
dc.identifier.urihttp://hdl.handle.net/20.500.14038/43801
dc.description.abstractInterleukin-10 (IL-10) is a pleiotropic cytokine that plays a pivotal role in the regulation of immune responses. Hence, we evaluated the effects of a recombinant adeno-associated viral vector 1 (rAAV1) encoding rat IL-10 (rAAV1-IL-10) in a rat model of kidney allograft rejection. Dark Agouti rat kidneys were transplanted into Wistar-Furth (WF) rats 8 weeks following a single intramuscular administration of either rAAV1-IL-10 or rAAV1-green fluorescence protein (GFP). Isografts (WF-WF) served as an additional experimental control. Both allograft and isograft recipients received daily cyclosporine (10 mg/kg) for 14 days after transplantation. Serum IL-10 levels increased at 8, 12 and 16 weeks following vector administration in rAAV1-IL-10-treated animals, but not in rAAV1-GFP and isograft groups. rAAV1-IL-10 treatment resulted in lower BUN and creatinine levels (p<0.001), as well as increased allograft survival rates from 22% to 90%. Allograft histological abnormalities were significantly attenuated in the rAAV1-IL-10-treated rats compared with those of rAAV1-GFP controls. Serum levels of proinflammatory cytokines such as growth-related oncogene were also significantly higher in the rAAV1-GFP group than in the rAAV1-IL-10 group. These data suggest delivery of IL-10 using a rAAV1 vector improves renal function and prolongs graft survival in a rat model of kidney transplant rejection.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=17456199&dopt=Abstract">Link to Article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1111/j.1600-6143.2007.01772.x
dc.subjectAnimals
dc.subjectBlood Urea Nitrogen
dc.subjectCreatinine
dc.subjectCytokines
dc.subjectDependovirus
dc.subjectFemale
dc.subject*Genetic Vectors
dc.subjectGraft Rejection
dc.subjectGraft Survival
dc.subjectGreen Fluorescent Proteins
dc.subjectInjections, Intramuscular
dc.subjectInterleukin-10
dc.subjectKidney
dc.subjectKidney Transplantation
dc.subjectModels, Animal
dc.subjectRats
dc.subjectRats, Inbred Strains
dc.subjectRats, Inbred WF
dc.subjectTransplantation, Homologous
dc.subjectAllergy and Immunology
dc.subjectGenetics and Genomics
dc.subjectPediatrics
dc.titleAdeno-associated viral vector-mediated interleukin-10 prolongs allograft survival in a rat kidney transplantation model
dc.typeJournal Article
dc.source.journaltitleAmerican journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant Surgeons
dc.source.volume7
dc.source.issue5
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/peds_pulmonary/17
dc.identifier.contextkey2441377
html.description.abstract<p>Interleukin-10 (IL-10) is a pleiotropic cytokine that plays a pivotal role in the regulation of immune responses. Hence, we evaluated the effects of a recombinant adeno-associated viral vector 1 (rAAV1) encoding rat IL-10 (rAAV1-IL-10) in a rat model of kidney allograft rejection. Dark Agouti rat kidneys were transplanted into Wistar-Furth (WF) rats 8 weeks following a single intramuscular administration of either rAAV1-IL-10 or rAAV1-green fluorescence protein (GFP). Isografts (WF-WF) served as an additional experimental control. Both allograft and isograft recipients received daily cyclosporine (10 mg/kg) for 14 days after transplantation. Serum IL-10 levels increased at 8, 12 and 16 weeks following vector administration in rAAV1-IL-10-treated animals, but not in rAAV1-GFP and isograft groups. rAAV1-IL-10 treatment resulted in lower BUN and creatinine levels (p<0.001), as well as increased allograft survival rates from 22% to 90%. Allograft histological abnormalities were significantly attenuated in the rAAV1-IL-10-treated rats compared with those of rAAV1-GFP controls. Serum levels of proinflammatory cytokines such as growth-related oncogene were also significantly higher in the rAAV1-GFP group than in the rAAV1-IL-10 group. These data suggest delivery of IL-10 using a rAAV1 vector improves renal function and prolongs graft survival in a rat model of kidney transplant rejection.</p>
dc.identifier.submissionpathpeds_pulmonary/17
dc.contributor.departmentDepartment of Pediatrics
dc.contributor.departmentGene Therapy Center
dc.source.pages1112-20


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