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dc.contributor.authorCarlson, David
dc.contributor.authorMcKeen, Elizabeth
dc.contributor.authorMitchell, Michael
dc.contributor.authorTorres, Brenda B.
dc.contributor.authorParad, Richard B.
dc.contributor.authorComeau, Anne Marie
dc.contributor.authorO'Sullivan, Brian P.
dc.date2022-08-11T08:10:14.000
dc.date.accessioned2022-08-23T17:00:34Z
dc.date.available2022-08-23T17:00:34Z
dc.date.issued2009-05-11
dc.date.submitted2012-01-11
dc.identifier.citationPediatr Pulmonol. 2009 May;44(5):497-502. <a href="http://dx.doi.org/10.1002/ppul.21029">Link to article on publisher's site</a>
dc.identifier.issn1099-0496 (Linking)
dc.identifier.doi10.1002/ppul.21029
dc.identifier.pmid19360845
dc.identifier.urihttp://hdl.handle.net/20.500.14038/43824
dc.description.abstractOBJECTIVE: The purpose of this preliminary study was to determine normal oropharyngeal flora in healthy, non-CF infants in order to help care givers better interpret culture results obtained from infants with CF. METHODS: Oropharyngeal cultures were obtained from 104 healthy infants <12 months old. Cultures were obtained using the same methods as for CF patients and were inoculated onto routine CF culture media. Approximately 20 infants from each of 5 age groups>(0-2 days, 3 days to <3 >months, 3 months to <6 >months, 6 months to <9 months or 9 months to><1 >year) were included in the well child sample. In addition, we reviewed serial results of upper airway cultures obtained during the first year of life from 20 CF-affected infants whose diagnosis was suggested by newborn screening. RESULTS: Well infants in the first 48 hr of life had very few pathogenic organisms found in their oropharyngeal cultures; 1/21 had S. aureus. Of the 83 samples from infants over 48 hr of age, we found that 27% (23/83) had S. aureus in their oropharyngeal cultures. Many infants had polymicrobial cultures. Eleven percent of culture samples had E. coli, E. cloacae, H. influenzae, or M. catarrhalis. Three of 83 cultures were positive for non-mucoid Ps. aeruginosa (3.6%), while 2 others were positive for Ps. putida. CONCLUSION: Healthy infants can have multiple gram-negative and gram-positive organisms recovered from their oropharynx. S. aureus and enteric gram-negative organisms, including non-mucoid Ps. aeruginosa, can be found in the oropharynx of well children up to 1 year of age. Care should be taken to not over interpret the presence of some of these organisms in the oropharyngeal cultures of asymptomatic CF infants.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=19360845&dopt=Abstract">Link to Article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1002/ppul.21029
dc.subjectCarrier State
dc.subjectCase-Control Studies
dc.subjectCystic Fibrosis
dc.subjectGram-Negative Aerobic Rods and Cocci
dc.subjectGram-Positive Cocci
dc.subjectHumans
dc.subjectInfant
dc.subjectInfant, Newborn
dc.subjectOropharynx
dc.subjectReference Values
dc.subjectAllergy and Immunology
dc.subjectPediatrics
dc.subjectRespiratory Tract Diseases
dc.titleOropharyngeal flora in healthy infants: observations and implications for cystic fibrosis care
dc.typeJournal Article
dc.source.journaltitlePediatric pulmonology
dc.source.volume44
dc.source.issue5
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/peds_pulmonary/38
dc.identifier.contextkey2441399
html.description.abstract<p>OBJECTIVE: The purpose of this preliminary study was to determine normal oropharyngeal flora in healthy, non-CF infants in order to help care givers better interpret culture results obtained from infants with CF.</p> <p>METHODS: Oropharyngeal cultures were obtained from 104 healthy infants <12 months old. Cultures were obtained using the same methods as for CF patients and were inoculated onto routine CF culture media. Approximately 20 infants from each of 5 age groups>(0-2 days, 3 days to <3 >months, 3 months to <6 >months, 6 months to <9 months or 9 months to><1 >year) were included in the well child sample. In addition, we reviewed serial results of upper airway cultures obtained during the first year of life from 20 CF-affected infants whose diagnosis was suggested by newborn screening.</p> <p>RESULTS: Well infants in the first 48 hr of life had very few pathogenic organisms found in their oropharyngeal cultures; 1/21 had S. aureus. Of the 83 samples from infants over 48 hr of age, we found that 27% (23/83) had S. aureus in their oropharyngeal cultures. Many infants had polymicrobial cultures. Eleven percent of culture samples had E. coli, E. cloacae, H. influenzae, or M. catarrhalis. Three of 83 cultures were positive for non-mucoid Ps. aeruginosa (3.6%), while 2 others were positive for Ps. putida.</p> <p>CONCLUSION: Healthy infants can have multiple gram-negative and gram-positive organisms recovered from their oropharynx. S. aureus and enteric gram-negative organisms, including non-mucoid Ps. aeruginosa, can be found in the oropharynx of well children up to 1 year of age. Care should be taken to not over interpret the presence of some of these organisms in the oropharyngeal cultures of asymptomatic CF infants.</p>
dc.identifier.submissionpathpeds_pulmonary/38
dc.contributor.departmentNew England Newborn Screening Program
dc.contributor.departmentDepartment of Pediatrics
dc.contributor.departmentDepartment of Laboratory Medicine
dc.source.pages497-502


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