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Gene transfer in the lung using recombinant adeno-associated virus

dc.contributor.authorGruntman, Alisha
dc.contributor.authorMueller, Christian
dc.contributor.authorFlotte, Terence R.
dc.contributor.authorGao, Guangping
dc.date2022-08-11T08:10:14.000
dc.date.accessioned2022-08-23T17:00:45Z
dc.date.available2022-08-23T17:00:45Z
dc.date.issued2012-08-01
dc.date.submitted2012-08-14
dc.identifier.citation<p>Curr Protoc Microbiol. 2012 Aug;Chapter 14:Unit14D.2. DOI: 10.1002/9780471729259.mc14d02s26</p>
dc.identifier.issn1934-8533
dc.identifier.doi10.1002/9780471729259.mc14d02s26
dc.identifier.pmid22875566
dc.identifier.urihttp://hdl.handle.net/20.500.14038/43860
dc.description.abstractAdeno-associated virus (AAV) is a small replication-deficient DNA virus belonging to the Parvovirinae family. It has a single-stranded ∼4.7-kb genome. Recombinant AAV (rAAV) is created by replacing the viral rep and cap genes with the transgene of interest along with promoter and polyadenylation sequences. The short viral inverted terminal repeats must remain intact for replication and packaging in production, as well as vector genome processing and persistence in the transduction process. The AAV capsid (serotype) determines the tissue tropism of the rAAV vector. In this unit we will discuss serotype selection for lung targeting along with the factors effecting efficient delivery of rAAV vectors to the murine lung. Detailed procedures for lung delivery (intranasal, orotracheal, and surgical tracheal injection), sample collection, and post-mortem tissue processing will be described. Curr. Protoc. Microbiol. 26:14D.2.1-14D.2.17. © 2012 by John Wiley & Sons, Inc.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=22875566&dopt=Abstract">Link to article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1002/9780471729259.mc14d02s26
dc.subjectDependovirus
dc.subjectGenetic Vectors
dc.subjectGene Therapy
dc.subjectTransgenes
dc.subjectGene Transfer Techniques
dc.subjectLung
dc.subjectAllergy and Immunology
dc.subjectGenetics and Genomics
dc.subjectMedical Genetics
dc.subjectPediatrics
dc.subjectRespiratory Tract Diseases
dc.titleGene transfer in the lung using recombinant adeno-associated virus
dc.typeBook Chapter
dc.source.booktitleCurrent Protocols in Microbiology
dc.source.volumeChapter 14
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/peds_pulmonary/71
dc.identifier.contextkey3212240
html.description.abstract<p>Adeno-associated virus (AAV) is a small replication-deficient DNA virus belonging to the Parvovirinae family. It has a single-stranded ∼4.7-kb genome. Recombinant AAV (rAAV) is created by replacing the viral rep and cap genes with the transgene of interest along with promoter and polyadenylation sequences. The short viral inverted terminal repeats must remain intact for replication and packaging in production, as well as vector genome processing and persistence in the transduction process. The AAV capsid (serotype) determines the tissue tropism of the rAAV vector. In this unit we will discuss serotype selection for lung targeting along with the factors effecting efficient delivery of rAAV vectors to the murine lung. Detailed procedures for lung delivery (intranasal, orotracheal, and surgical tracheal injection), sample collection, and post-mortem tissue processing will be described. Curr. Protoc. Microbiol. 26:14D.2.1-14D.2.17. © 2012 by John Wiley & Sons, Inc.</p>
dc.identifier.submissionpathpeds_pulmonary/71
dc.contributor.departmentDepartment of Microbiology and Physiological Systems
dc.contributor.departmentDepartment of Pediatrics
dc.contributor.departmentGene Therapy Center
dc.source.pagesUnit14D.2


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