C. elegans PAT-9 is a nuclear zinc finger protein critical for the assembly of muscle attachments
dc.contributor.author | Liu, Qian | |
dc.contributor.author | Jones, Takako I. | |
dc.contributor.author | Bachmann, Rebecca A. | |
dc.contributor.author | Meghpara, Mitchell | |
dc.contributor.author | Rogowski, Lauren | |
dc.contributor.author | Williams, Benjamin D. | |
dc.contributor.author | Jones, Peter L. | |
dc.date | 2022-08-11T08:10:14.000 | |
dc.date.accessioned | 2022-08-23T17:00:50Z | |
dc.date.available | 2022-08-23T17:00:50Z | |
dc.date.issued | 2012-05-22 | |
dc.date.submitted | 2014-12-04 | |
dc.identifier.citation | Cell Biosci. 2012 May 22;2(1):18. doi: 10.1186/2045-3701-2-18. <a href="http://dx.doi.org/10.1186/2045-3701-2-18">Link to article on publisher's site</a> | |
dc.identifier.issn | 2045-3701 (Linking) | |
dc.identifier.doi | 10.1186/2045-3701-2-18 | |
dc.identifier.pmid | 22616817 | |
dc.identifier.uri | http://hdl.handle.net/20.500.14038/43878 | |
dc.description | <p>At the time of publication, Peter Jones and Takako Jones were not yet affiliated with the University of Massachusetts Medical School.</p> | |
dc.description.abstract | BACKGROUND: Caenorhabditis elegans sarcomeres have been studied extensively utilizing both forward and reverse genetic techniques to provide insight into muscle development and the mechanisms behind muscle contraction. A previous genetic screen investigating early muscle development produced 13 independent mutant genes exhibiting a Pat (paralyzed and arrested elongation at the two-fold length of embryonic development) muscle phenotype. This study reports the identification and characterization of one of those genes, pat-9. RESULTS: Positional cloning, reverse genetics, and plasmid rescue experiments were used to identify the predicted C. elegans gene T27B1.2 (recently named ztf-19) as the pat-9 gene. Analysis of pat-9 showed it is expressed early in development and within body wall muscle lineages, consistent with a role in muscle development and producing a Pat phenotype. However, unlike most of the other known Pat gene family members, which encode structural components of muscle attachment sites, PAT-9 is an exclusively nuclear protein. Analysis of the predicted PAT-9 amino acid sequence identified one putative nuclear localization domain and three C2H2 zinc finger domains. Both immunocytochemistry and PAT-9::GFP fusion expression confirm that PAT-9 is primarily a nuclear protein and chromatin immunoprecipitation (ChIP) experiments showed that PAT-9 is present on certain gene promoters. CONCLUSIONS: We have shown that the T27B1.2 gene is pat-9. Considering the Pat-9 mutant phenotype shows severely disrupted muscle attachment sites despite PAT-9 being a nuclear zinc finger protein and not a structural component of muscle attachment sites, we propose that PAT-9 likely functions in the regulation of gene expression for some necessary structural or regulatory component(s) of the muscle attachment sites. | |
dc.language.iso | en_US | |
dc.relation | <a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=22616817&dopt=Abstract">Link to Article in PubMed</a> | |
dc.rights | <p>Copyright ©2012 Liu et al.; licensee BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (<a href="http://creativecommons.org/licenses/by/2.0">http://creativecommons.org/licenses/by/2.0</a>), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.</p> | |
dc.subject | Sarcomere | |
dc.subject | Muscle | |
dc.subject | Zinc finger | |
dc.subject | Pat | |
dc.subject | Cell Biology | |
dc.subject | Developmental Biology | |
dc.subject | Molecular Biology | |
dc.subject | Molecular Genetics | |
dc.subject | Musculoskeletal Diseases | |
dc.subject | Nervous System Diseases | |
dc.title | C. elegans PAT-9 is a nuclear zinc finger protein critical for the assembly of muscle attachments | |
dc.type | Journal Article | |
dc.source.journaltitle | Cell and bioscience | |
dc.source.volume | 2 | |
dc.source.issue | 1 | |
dc.identifier.legacyfulltext | https://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=1017&context=peterjones&unstamped=1 | |
dc.identifier.legacycoverpage | https://escholarship.umassmed.edu/peterjones/18 | |
dc.legacy.embargo | 2014-12-11T00:00:00-08:00 | |
dc.identifier.contextkey | 6422502 | |
refterms.dateFOA | 2022-08-23T17:00:50Z | |
html.description.abstract | <p>BACKGROUND: Caenorhabditis elegans sarcomeres have been studied extensively utilizing both forward and reverse genetic techniques to provide insight into muscle development and the mechanisms behind muscle contraction. A previous genetic screen investigating early muscle development produced 13 independent mutant genes exhibiting a Pat (paralyzed and arrested elongation at the two-fold length of embryonic development) muscle phenotype. This study reports the identification and characterization of one of those genes, pat-9.</p> <p>RESULTS: Positional cloning, reverse genetics, and plasmid rescue experiments were used to identify the predicted C. elegans gene T27B1.2 (recently named ztf-19) as the pat-9 gene. Analysis of pat-9 showed it is expressed early in development and within body wall muscle lineages, consistent with a role in muscle development and producing a Pat phenotype. However, unlike most of the other known Pat gene family members, which encode structural components of muscle attachment sites, PAT-9 is an exclusively nuclear protein. Analysis of the predicted PAT-9 amino acid sequence identified one putative nuclear localization domain and three C2H2 zinc finger domains. Both immunocytochemistry and PAT-9::GFP fusion expression confirm that PAT-9 is primarily a nuclear protein and chromatin immunoprecipitation (ChIP) experiments showed that PAT-9 is present on certain gene promoters.</p> <p>CONCLUSIONS: We have shown that the T27B1.2 gene is pat-9. Considering the Pat-9 mutant phenotype shows severely disrupted muscle attachment sites despite PAT-9 being a nuclear zinc finger protein and not a structural component of muscle attachment sites, we propose that PAT-9 likely functions in the regulation of gene expression for some necessary structural or regulatory component(s) of the muscle attachment sites.</p> | |
dc.identifier.submissionpath | peterjones/18 | |
dc.contributor.department | Department of Cell and Developmental Biology | |
dc.source.pages | 18 |