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dc.contributor.authorWalhout, Albertha J. M.
dc.contributor.authorBoulton, Simon J.
dc.date2022-08-11T08:10:14.000
dc.date.accessioned2022-08-23T17:00:53Z
dc.date.available2022-08-23T17:00:53Z
dc.date.issued2006-02-13
dc.date.submitted2009-11-19
dc.identifier.citationWalhout, A.J.M. and Boulton, S.J. Biochemistry and molecular biology (February 13, 2006), WormBook, ed. The C. elegans Research Community, WormBook, <a href="http://dx.doi.org/10.1895/wormbook.1.86.1">http://dx.doi.org/110.1895/wormbook.1.86.1</a>, <a href="http://www.wormbook.org">http://www.wormbook.org</a>.
dc.identifier.doi110.1895/wormbook.1.86.1
dc.identifier.urihttp://hdl.handle.net/20.500.14038/43888
dc.description.abstractSeveral forward and reverse proteomic approaches are available that can be used to identify interaction partners for a protein of interest. Here we provide methods for identifying interacting partners by the yeast two-hybrid system (a reverse proteomic method) and by tandem immuno-affinity purification of protein complexes combined with mass spectrometry (a forward proteomic method).
dc.language.isoen_US
dc.subjectCaenorhabditis elegans
dc.subjectCaenorhabditis elegans Proteins
dc.subjectProtein Interaction Mapping
dc.subjectTwo-Hybrid System Techniques
dc.subjectTandem Mass Spectrometry
dc.subjectGenetics and Genomics
dc.titleBiochemistry and Molecular Biology
dc.typeBook Chapter
dc.source.booktitleThe C. elegans Research Community, WormBook
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=1000&amp;context=pgfe_pp&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/pgfe_pp/1
dc.identifier.contextkey1068366
refterms.dateFOA2022-08-23T17:00:53Z
html.description.abstract<p>Several forward and reverse proteomic approaches are available that can be used to identify interaction partners for a protein of interest. Here we provide methods for identifying interacting partners by the yeast two-hybrid system (a reverse proteomic method) and by tandem immuno-affinity purification of protein complexes combined with mass spectrometry (a forward proteomic method).</p>
dc.identifier.submissionpathpgfe_pp/1
dc.contributor.departmentProgram in Molecular Medicine
dc.contributor.departmentProgram in Gene Function and Expression


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