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    Mapping chromatin interactions by chromosome conformation capture

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    Authors
    Miele, Adriana
    Gheldof, Nele
    Tabuchi, Tomoko M.
    Dostie, Josee
    Dekker, Job
    UMass Chan Affiliations
    Program in Gene Function and Expression
    Document Type
    Book Chapter
    Publication Date
    2006-05-12
    Keywords
    Cells, Cultured
    Chromatin
    Chromosomes
    Genomics
    Models, Biological
    Nucleic Acid Conformation
    Saccharomyces cerevisiae
    Genetics and Genomics
    
    Metadata
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    Link to Full Text
    http://dx.doi.org/10.1002/0471142727.mb2111s74
    Abstract
    Chromosome conformation capture (3C) is one of the only techniques that allows for analysis of an intermediate level of chromosome structure ranging from a few to hundreds of kilobases, a level most relevant for gene regulation. The 3C technique is used to detect physical interactions between sequence elements that are located on the same or on different chromosomes. For instance, physical interactions between distant enhancers and target genes can be measured. The 3C assay uses formaldehyde cross-linking to trap connections between chromatin segments that can, after a number of manipulations, be detected by PCR. This unit describes detailed protocols for performing 3C with yeast Saccharomyces cerevisiae and mammalian cells.
    Source
    Curr Protoc Mol Biol. 2006 May;Chapter 21:Unit 21.11. Link to article on publisher's site
    DOI
    10.1002/0471142727.mb2111s74
    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/43907
    PubMed ID
    18265379
    Related Resources
    Link to Article in PubMed
    ae974a485f413a2113503eed53cd6c53
    10.1002/0471142727.mb2111s74
    Scopus Count
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    UMass Chan Faculty and Researcher Publications

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