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dc.contributor.authorMiele, Adriana
dc.contributor.authorGheldof, Nele
dc.contributor.authorTabuchi, Tomoko M.
dc.contributor.authorDostie, Josee
dc.contributor.authorDekker, Job
dc.date2022-08-11T08:10:15.000
dc.date.accessioned2022-08-23T17:00:59Z
dc.date.available2022-08-23T17:00:59Z
dc.date.issued2006-05-12
dc.date.submitted2011-04-19
dc.identifier.citationCurr Protoc Mol Biol. 2006 May;Chapter 21:Unit 21.11. <a href="http://dx.doi.org/10.1002/0471142727.mb2111s74">Link to article on publisher's site</a>
dc.identifier.issn1934-3647 (Linking)
dc.identifier.doi10.1002/0471142727.mb2111s74
dc.identifier.pmid18265379
dc.identifier.urihttp://hdl.handle.net/20.500.14038/43907
dc.description.abstractChromosome conformation capture (3C) is one of the only techniques that allows for analysis of an intermediate level of chromosome structure ranging from a few to hundreds of kilobases, a level most relevant for gene regulation. The 3C technique is used to detect physical interactions between sequence elements that are located on the same or on different chromosomes. For instance, physical interactions between distant enhancers and target genes can be measured. The 3C assay uses formaldehyde cross-linking to trap connections between chromatin segments that can, after a number of manipulations, be detected by PCR. This unit describes detailed protocols for performing 3C with yeast Saccharomyces cerevisiae and mammalian cells.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=18265379&dopt=Abstract">Link to Article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1002/0471142727.mb2111s74
dc.subjectCells, Cultured
dc.subjectChromatin
dc.subjectChromosomes
dc.subjectGenomics
dc.subjectModels, Biological
dc.subjectNucleic Acid Conformation
dc.subjectSaccharomyces cerevisiae
dc.subjectGenetics and Genomics
dc.titleMapping chromatin interactions by chromosome conformation capture
dc.typeBook Chapter
dc.source.booktitleCurrent protocols in molecular biology / edited by Frederick M. Ausubel ... [et al.]
dc.source.volumeChapter 21
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/pgfe_pp/118
dc.identifier.contextkey1946773
html.description.abstract<p>Chromosome conformation capture (3C) is one of the only techniques that allows for analysis of an intermediate level of chromosome structure ranging from a few to hundreds of kilobases, a level most relevant for gene regulation. The 3C technique is used to detect physical interactions between sequence elements that are located on the same or on different chromosomes. For instance, physical interactions between distant enhancers and target genes can be measured. The 3C assay uses formaldehyde cross-linking to trap connections between chromatin segments that can, after a number of manipulations, be detected by PCR. This unit describes detailed protocols for performing 3C with yeast Saccharomyces cerevisiae and mammalian cells.</p>
dc.identifier.submissionpathpgfe_pp/118
dc.contributor.departmentProgram in Gene Function and Expression
dc.source.pagesUnit 21.11


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