The three 'C' s of chromosome conformation capture: controls, controls, controls
Authors
Dekker, JobUMass Chan Affiliations
Program in Gene Function and ExpressionDocument Type
Journal ArticlePublication Date
2006-01-22Keywords
ChromosomesDNA
Gene Expression Regulation
Genes
Genomics
Nucleic Acid Conformation
Polymerase Chain Reaction
*Regulatory Elements, Transcriptional
Reproducibility of Results
Genetics and Genomics
Metadata
Show full item recordAbstract
Transcription regulation in higher eukaryotes is controlled by regulatory elements such as enhancers that are recognized by transcription factors. In many cases regulatory elements can be located at distances up to several megabases from their target genes. Recent evidence shows that long-range control of gene expression can be mediated through direct physical interactions between genes and these regulatory elements. Such looping interactions can be detected using the chromosome conformation capture (3C) methodology. Although 3C is experimentally straightforward, to draw meaningful conclusions one must carefully design 3C experiments and implement the conscientious use of controls. The general guidelines presented here should help experimental design and minimize misinterpretation of 3C experiments.Source
Nat Methods. 2006 Jan;3(1):17-21. Link to article on publisher's siteDOI
10.1038/nmeth823Permanent Link to this Item
http://hdl.handle.net/20.500.14038/43908PubMed ID
16369547Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1038/nmeth823