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    The three 'C' s of chromosome conformation capture: controls, controls, controls

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    Authors
    Dekker, Job
    UMass Chan Affiliations
    Program in Gene Function and Expression
    Document Type
    Journal Article
    Publication Date
    2006-01-22
    Keywords
    Chromosomes
    DNA
    Gene Expression Regulation
    Genes
    Genomics
    Nucleic Acid Conformation
    Polymerase Chain Reaction
    *Regulatory Elements, Transcriptional
    Reproducibility of Results
    Genetics and Genomics
    
    Metadata
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    Link to Full Text
    http://dx.doi.org/10.1038/nmeth823
    Abstract
    Transcription regulation in higher eukaryotes is controlled by regulatory elements such as enhancers that are recognized by transcription factors. In many cases regulatory elements can be located at distances up to several megabases from their target genes. Recent evidence shows that long-range control of gene expression can be mediated through direct physical interactions between genes and these regulatory elements. Such looping interactions can be detected using the chromosome conformation capture (3C) methodology. Although 3C is experimentally straightforward, to draw meaningful conclusions one must carefully design 3C experiments and implement the conscientious use of controls. The general guidelines presented here should help experimental design and minimize misinterpretation of 3C experiments.
    Source
    Nat Methods. 2006 Jan;3(1):17-21. Link to article on publisher's site
    DOI
    10.1038/nmeth823
    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/43908
    PubMed ID
    16369547
    Related Resources
    Link to Article in PubMed
    ae974a485f413a2113503eed53cd6c53
    10.1038/nmeth823
    Scopus Count
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    UMass Chan Faculty and Researcher Publications

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