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    Histone deposition protein Asf1 maintains DNA replisome integrity and interacts with replication factor C

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    Authors
    Franco, Alexa A.
    Lam, Wendy M.
    Burgers, Peter M.
    Kaufman, Paul D.
    UMass Chan Affiliations
    Program in Gene Function and Expression
    Document Type
    Journal Article
    Publication Date
    2005-05-20
    Keywords
    Cell Cycle Proteins
    *DNA Replication
    Histones
    Protein Binding
    S Phase
    Genetics and Genomics
    
    Metadata
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    Link to Full Text
    http://dx.doi.org/10.1101/gad.1305005
    Abstract
    Chromatin assembly and DNA replication are temporally coupled, and DNA replication in the absence of histone synthesis causes inviability. Here we demonstrate that chromatin assembly factor Asf1 also affects DNA replication. In budding yeast cells lacking Asf1, the amounts of several DNA replication proteins, including replication factor C (RFC), proliferating cell nuclear antigen (PCNA), and DNA polymerase epsilon (Pol epsilon), are reduced at stalled replication forks. In contrast, DNA polymerase alpha (Pol alpha) accumulates to higher than normal levels at stalled forks in asf1Delta cells. Using purified, recombinant proteins, we demonstrate that RFC directly binds Asf1 and can recruit Asf1 to DNA molecules in vitro. We conclude that histone chaperone protein Asf1 maintains a subset of replication elongation factors at stalled replication forks and directly interacts with the replication machinery.
    Source
    Genes Dev. 2005 Jun 1;19(11):1365-75. Epub 2005 May 18. Link to article on publisher's site
    DOI
    10.1101/gad.1305005
    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/43920
    PubMed ID
    15901673
    Related Resources
    Link to Article in PubMed
    ae974a485f413a2113503eed53cd6c53
    10.1101/gad.1305005
    Scopus Count
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    UMass Chan Faculty and Researcher Publications

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