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    Formation of MacroH2A-containing senescence-associated heterochromatin foci and senescence driven by ASF1a and HIRA

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    Authors
    Zhang, Rugang
    Poustovoitov, Maxim V.
    Ye, Xiaofen
    Santos, Hidelita A.
    Chen, Wei
    Daganzo, Sally M.
    Erzberger, Jan P.
    Serebriiskii, Ilya G.
    Canutescu, Adrian A.
    Dunbrack, Roland L.
    Pehrson, John R.
    Berger, James M.
    Kaufman, Paul D.
    Adams, Peter D.
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    UMass Chan Affiliations
    Program in Gene Function and Expression
    Document Type
    Journal Article
    Publication Date
    2005-01-29
    Keywords
    Amino Acid Sequence
    Blotting, Western
    Cell Aging
    Cell Count
    Cell Cycle
    Cell Cycle Proteins
    Cell Line
    Chromosomal Proteins, Non-Histone
    Dosage Compensation, Genetic
    Gene Expression Regulation
    Heterochromatin
    Histones
    Immunohistochemistry
    Immunoprecipitation
    Indoles
    Neoplasm Proteins
    Nuclear Proteins
    Recombinant Fusion Proteins
    Repressor Proteins
    Time Factors
    Transcription Factors
    Transfection
    Tumor Suppressor Proteins
    ras Proteins
    Genetics and Genomics
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    http://dx.doi.org/10.1016/j.devcel.2004.10.019
    Abstract
    In senescent cells, specialized domains of transcriptionally silent senescence-associated heterochromatic foci (SAHF), containing heterochromatin proteins such as HP1, are thought to repress expression of proliferation-promoting genes. We have investigated the composition and mode of assembly of SAHF and its contribution to cell cycle exit. SAHF is enriched in a transcription-silencing histone H2A variant, macroH2A. As cells approach senescence, a known chromatin regulator, HIRA, enters PML nuclear bodies, where it transiently colocalizes with HP1 proteins, prior to incorporation of HP1 proteins into SAHF. A physical complex containing HIRA and another chromatin regulator, ASF1a, is rate limiting for formation of SAHF and onset of senescence, and ASF1a is required for formation of SAHF and efficient senescence-associated cell cycle exit. These data indicate that HIRA and ASF1a drive formation of macroH2A-containing SAHF and senescence-associated cell cycle exit, via a pathway that appears to depend on flux of heterochromatic proteins through PML bodies.
    Source
    Dev Cell. 2005 Jan;8(1):19-30. Link to article on publisher's site
    DOI
    10.1016/j.devcel.2004.10.019
    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/43923
    PubMed ID
    15621527
    Related Resources
    Link to Article in PubMed
    ae974a485f413a2113503eed53cd6c53
    10.1016/j.devcel.2004.10.019
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