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    Lhx9 and lhx9alpha: differential biochemical properties and effects on neuronal differentiation

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    Authors
    Molle, Bertrand
    Pere, Stephane
    Failli, Vieri
    Bach, Ingolf
    Retaux, Sylvie
    UMass Chan Affiliations
    Program in Molecular Medicine
    Programs in Gene Function and Expression
    Document Type
    Journal Article
    Publication Date
    2004-12-09
    Keywords
    Animals
    Cell Differentiation
    Electrophoretic Mobility Shift Assay
    Fluorescent Antibody Technique
    Homeodomain Proteins
    Neurons
    PC12 Cells
    Protein Isoforms
    Rats
    Transcription, Genetic
    Genetics and Genomics
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    Link to Full Text
    http://dx.doi.org/10.1089/dna.2004.23.761
    Abstract
    The Lhx9 LIM-homeodomain transcription factor and its truncated isoform Lhx9alpha are generated by alternative splicing of the Lhx9 gene. Here we investigated the differential functional properties of these two isoforms. Lhx9alpha, which lacks parts of the homeodomain, was unable to bind DNA in EMSA experiments, but was able to associate with CLIM cofactors in GST pull-down assays. In transfection experiments in PC12 cells, Lhx9alpha fusion constructs systematically showed a nuclear localization, as opposed to Lhx9 fusion constructs, which also localized to the cytoplasm. Moreover, Lhx9 increased NGF-induced neuronal differentiation of PC12 cells. Lhx9alpha, on the other hand, did not significantly increase neuronal differentiation but had an effect on the morphology of PC12 cells. Finally, as tested by RT-PCR experiments on transfected PC12 cells, Lhx9 was not able to induce the transcription of Lhx9alpha. Our results show significantly different functional properties for Lhx9 and Lhx9alpha, and suggest that Lhx9alpha can compete away limiting amounts of nuclear CLIM cofactors. Thus, Lhx9 and Lhx9alpha isoforms could be implicated in regulating various aspects of neuronal differentiation.
    Source
    Bertrand Mollé, Stéphane Père, Vieri Failli, Ingolf Bach, Sylvie Rétaux. DNA and Cell Biology. November 2004, 23(11): 761-768. doi:10.1089/dna.2004.23.761.
    DOI
    10.1089/dna.2004.23.761
    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/43926
    PubMed ID
    15585134
    Related Resources
    Link to Article in PubMed
    Rights
    Copyright © 2004 Mary Ann Liebert, Inc. Link to article on publisher's site.
    ae974a485f413a2113503eed53cd6c53
    10.1089/dna.2004.23.761
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