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dc.contributor.authorMolle, Bertrand
dc.contributor.authorPere, Stephane
dc.contributor.authorFailli, Vieri
dc.contributor.authorBach, Ingolf
dc.contributor.authorRetaux, Sylvie
dc.date2022-08-11T08:10:15.000
dc.date.accessioned2022-08-23T17:01:04Z
dc.date.available2022-08-23T17:01:04Z
dc.date.issued2004-12-09
dc.date.submitted2011-04-19
dc.identifier.citationBertrand Mollé, Stéphane Père, Vieri Failli, Ingolf Bach, Sylvie Rétaux. DNA and Cell Biology. November 2004, 23(11): 761-768. doi:10.1089/dna.2004.23.761.
dc.identifier.issn1044-5498 (Linking)
dc.identifier.doi10.1089/dna.2004.23.761
dc.identifier.pmid15585134
dc.identifier.urihttp://hdl.handle.net/20.500.14038/43926
dc.description.abstractThe Lhx9 LIM-homeodomain transcription factor and its truncated isoform Lhx9alpha are generated by alternative splicing of the Lhx9 gene. Here we investigated the differential functional properties of these two isoforms. Lhx9alpha, which lacks parts of the homeodomain, was unable to bind DNA in EMSA experiments, but was able to associate with CLIM cofactors in GST pull-down assays. In transfection experiments in PC12 cells, Lhx9alpha fusion constructs systematically showed a nuclear localization, as opposed to Lhx9 fusion constructs, which also localized to the cytoplasm. Moreover, Lhx9 increased NGF-induced neuronal differentiation of PC12 cells. Lhx9alpha, on the other hand, did not significantly increase neuronal differentiation but had an effect on the morphology of PC12 cells. Finally, as tested by RT-PCR experiments on transfected PC12 cells, Lhx9 was not able to induce the transcription of Lhx9alpha. Our results show significantly different functional properties for Lhx9 and Lhx9alpha, and suggest that Lhx9alpha can compete away limiting amounts of nuclear CLIM cofactors. Thus, Lhx9 and Lhx9alpha isoforms could be implicated in regulating various aspects of neuronal differentiation.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=15585134&dopt=Abstract">Link to Article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1089/dna.2004.23.761
dc.rightsCopyright © 2004 Mary Ann Liebert, Inc. <a href="http://dx.doi.org/10.1089/dna.2004.23.761">Link to article on publisher's site</a>.
dc.subjectAnimals
dc.subjectCell Differentiation
dc.subjectElectrophoretic Mobility Shift Assay
dc.subjectFluorescent Antibody Technique
dc.subjectHomeodomain Proteins
dc.subjectNeurons
dc.subjectPC12 Cells
dc.subjectProtein Isoforms
dc.subjectRats
dc.subjectTranscription, Genetic
dc.subjectGenetics and Genomics
dc.titleLhx9 and lhx9alpha: differential biochemical properties and effects on neuronal differentiation
dc.typeJournal Article
dc.source.journaltitleDNA and cell biology
dc.source.volume23
dc.source.issue11
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=1137&amp;context=pgfe_pp&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/pgfe_pp/137
dc.identifier.contextkey1946792
refterms.dateFOA2022-08-23T17:01:04Z
html.description.abstract<p>The Lhx9 LIM-homeodomain transcription factor and its truncated isoform Lhx9alpha are generated by alternative splicing of the Lhx9 gene. Here we investigated the differential functional properties of these two isoforms. Lhx9alpha, which lacks parts of the homeodomain, was unable to bind DNA in EMSA experiments, but was able to associate with CLIM cofactors in GST pull-down assays. In transfection experiments in PC12 cells, Lhx9alpha fusion constructs systematically showed a nuclear localization, as opposed to Lhx9 fusion constructs, which also localized to the cytoplasm. Moreover, Lhx9 increased NGF-induced neuronal differentiation of PC12 cells. Lhx9alpha, on the other hand, did not significantly increase neuronal differentiation but had an effect on the morphology of PC12 cells. Finally, as tested by RT-PCR experiments on transfected PC12 cells, Lhx9 was not able to induce the transcription of Lhx9alpha. Our results show significantly different functional properties for Lhx9 and Lhx9alpha, and suggest that Lhx9alpha can compete away limiting amounts of nuclear CLIM cofactors. Thus, Lhx9 and Lhx9alpha isoforms could be implicated in regulating various aspects of neuronal differentiation.</p>
dc.identifier.submissionpathpgfe_pp/137
dc.contributor.departmentProgram in Molecular Medicine
dc.contributor.departmentPrograms in Gene Function and Expression
dc.source.pages761-8


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