AuthorsBrady, Siobhan M.
Martinez, Natalia Julia
Yi, Charles S.
Walhout, Albertha J. M.
Benfey, Philip N.
UMass Chan AffiliationsProgram in Molecular Medicine
Program in Gene Function and Expression
Document TypeJournal Article
Gene Expression Profiling
Gene Regulatory Networks
Reproducibility of Results
Two-Hybrid System Techniques
Genetics and Genomics
MetadataShow full item record
AbstractTightly controlled gene expression is a hallmark of multicellular development and is accomplished by transcription factors (TFs) and microRNAs (miRNAs). Although many studies have focused on identifying downstream targets of these molecules, less is known about the factors that regulate their differential expression. We used data from high spatial resolution gene expression experiments and yeast one-hybrid (Y1H) and two-hybrid (Y2H) assays to delineate a subset of interactions occurring within a gene regulatory network (GRN) that determines tissue-specific TF and miRNA expression in plants. We find that upstream TFs are expressed in more diverse cell types than their targets and that promoters that are bound by a relatively large number of TFs correspond to key developmental regulators. The regulatory consequence of many TFs for their target was experimentally determined using genetic analysis. Remarkably, molecular phenotypes were identified for 65% of the TFs, but morphological phenotypes were associated with only 16%. This indicates that the GRN is robust, and that gene expression changes may be canalized or buffered.
SourceMol Syst Biol. 2011 Jan 18;7:459. Link to article on publisher's site
Permanent Link to this Itemhttp://hdl.handle.net/20.500.14038/43959
Related ResourcesLink to Article in PubMed
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