The three-dimensional architecture of a bacterial genome and its alteration by genetic perturbation
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Authors
Umbarger, Mark A.Toro, Esteban
Wright, Matthew A.
Porreca, Gregory J.
Bau, Davide
Hong, Sun-Hae
Fero, Michael J.
Zhu, Lihua Julie
Marti-Renom, Marc A.
McAdams, Harley H.
Shapiro, Lucy
Dekker, Job
Church, George M.
UMass Chan Affiliations
Program in Systems BiologyDepartment of Biochemistry and Molecular Pharmacology
Program in Gene Function and Expression
Document Type
Journal ArticlePublication Date
2011-10-25Keywords
Caulobacter crescentusGenome, Bacterial
Chromosomes, Bacterial
Imaging, Three-Dimensional
Genetics and Genomics
Metadata
Show full item recordAbstract
We have determined the three-dimensional (3D) architecture of the Caulobacter crescentus genome by combining genome-wide chromatin interaction detection, live-cell imaging, and computational modeling. Using chromosome conformation capture carbon copy (5C), we derive approximately 13 kb resolution 3D models of the Caulobacter genome. The resulting models illustrate that the genome is ellipsoidal with periodically arranged arms. The parS sites, a pair of short contiguous sequence elements known to be involved in chromosome segregation, are positioned at one pole, where they anchor the chromosome to the cell and contribute to the formation of a compact chromatin conformation. Repositioning these elements resulted in rotations of the chromosome that changed the subcellular positions of most genes. Such rotations did not lead to large-scale changes in gene expression, indicating that genome folding does not strongly affect gene regulation. Collectively, our data suggest that genome folding is globally dictated by the parS sites and chromosome segregation.Source
Mol Cell. 2011 Oct 21;44(2):252-64. Link to article on publisher's siteDOI
10.1016/j.molcel.2011.09.010Permanent Link to this Item
http://hdl.handle.net/20.500.14038/43960PubMed ID
22017872Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1016/j.molcel.2011.09.010