Rayla, Amy L.
Wolfe, Scot A.
Christensen, Ryan G.
Stormo, Gary D.
UMass Chan AffiliationsDepartment of Biochemistry and Molecular Pharmacology
Program in Gene Function and Expression
Document TypeJournal Article
MetadataShow full item record
AbstractThe widespread use of zinc-finger nucleases (ZFNs) for genome engineering is hampered by the fact that only a subset of sequences can be efficiently recognized using published finger archives. We describe a set of validated two-finger modules that complement existing finger archives and expand the range of ZFN-accessible sequences threefold. Using this archive, we introduced lesions at 9 of 11 target sites in the zebrafish genome.
SourceNat Methods. 2012 Apr 29. doi: 10.1038/nmeth.1994. [Epub ahead of print]
Permanent Link to this Itemhttp://hdl.handle.net/20.500.14038/43975
Related ResourcesLink to article in PubMed