Authors
Gupta, AnkitRayla, Amy L.
Lakshmanan, Abirami
Wolfe, Scot A.
Christensen, Ryan G.
Stormo, Gary D.
UMass Chan Affiliations
Department of Biochemistry and Molecular PharmacologyProgram in Gene Function and Expression
Document Type
Journal ArticlePublication Date
2012-04-29
Metadata
Show full item recordAbstract
The widespread use of zinc-finger nucleases (ZFNs) for genome engineering is hampered by the fact that only a subset of sequences can be efficiently recognized using published finger archives. We describe a set of validated two-finger modules that complement existing finger archives and expand the range of ZFN-accessible sequences threefold. Using this archive, we introduced lesions at 9 of 11 target sites in the zebrafish genome.Source
Nat Methods. 2012 Apr 29. doi: 10.1038/nmeth.1994. [Epub ahead of print]DOI
10.1038/nmeth.1994Permanent Link to this Item
http://hdl.handle.net/20.500.14038/43975PubMed ID
22543349Related Resources
Link to article in PubMedae974a485f413a2113503eed53cd6c53
10.1038/nmeth.1994