An optimized two-finger archive for ZFN-mediated gene targeting
| dc.contributor.author | Gupta, Ankit | |
| dc.contributor.author | Rayla, Amy L. | |
| dc.contributor.author | Lakshmanan, Abirami | |
| dc.contributor.author | Wolfe, Scot A. | |
| dc.contributor.author | Christensen, Ryan G. | |
| dc.contributor.author | Stormo, Gary D. | |
| dc.date | 2022-08-11T08:10:15.000 | |
| dc.date.accessioned | 2022-08-23T17:01:17Z | |
| dc.date.available | 2022-08-23T17:01:17Z | |
| dc.date.issued | 2012-04-29 | |
| dc.date.submitted | 2012-05-21 | |
| dc.identifier.citation | Nat Methods. 2012 Apr 29. doi: 10.1038/nmeth.1994. [Epub ahead of print] | |
| dc.identifier.issn | 1548-7091 (Linking) | |
| dc.identifier.doi | 10.1038/nmeth.1994 | |
| dc.identifier.pmid | 22543349 | |
| dc.identifier.uri | http://hdl.handle.net/20.500.14038/43975 | |
| dc.description.abstract | The widespread use of zinc-finger nucleases (ZFNs) for genome engineering is hampered by the fact that only a subset of sequences can be efficiently recognized using published finger archives. We describe a set of validated two-finger modules that complement existing finger archives and expand the range of ZFN-accessible sequences threefold. Using this archive, we introduced lesions at 9 of 11 target sites in the zebrafish genome. | |
| dc.language.iso | en_US | |
| dc.relation | <a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=22543349&dopt=Abstract">Link to article in PubMed</a> | |
| dc.relation.url | http://dx.doi.org/10.1038/nmeth.1994 | |
| dc.subject | Zinc Fingers | |
| dc.subject | Gene Targeting | |
| dc.subject | Genetics and Genomics | |
| dc.title | An optimized two-finger archive for ZFN-mediated gene targeting | |
| dc.type | Journal Article | |
| dc.source.journaltitle | Nature methods | |
| dc.identifier.legacycoverpage | https://escholarship.umassmed.edu/pgfe_pp/186 | |
| dc.identifier.contextkey | 2878935 | |
| html.description.abstract | <p>The widespread use of zinc-finger nucleases (ZFNs) for genome engineering is hampered by the fact that only a subset of sequences can be efficiently recognized using published finger archives. We describe a set of validated two-finger modules that complement existing finger archives and expand the range of ZFN-accessible sequences threefold. Using this archive, we introduced lesions at 9 of 11 target sites in the zebrafish genome.</p> | |
| dc.identifier.submissionpath | pgfe_pp/186 | |
| dc.contributor.department | Department of Biochemistry and Molecular Pharmacology | |
| dc.contributor.department | Program in Gene Function and Expression |