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dc.contributor.authorDupuy, Denis
dc.contributor.authorLi, Qian-Ru
dc.contributor.authorDeplancke, Bart
dc.contributor.authorBoxem, Mike
dc.contributor.authorHao, Tong
dc.contributor.authorLamesch, Philippe
dc.contributor.authorSequerra, Reynaldo
dc.contributor.authorBosak, Stephanie
dc.contributor.authorDoucette-Stamm, Lynn
dc.contributor.authorHope, Ian A.
dc.contributor.authorHill, David E.
dc.contributor.authorWalhout, Albertha J. M.
dc.contributor.authorVidal, Marc
dc.date2022-08-11T08:10:15.000
dc.date.accessioned2022-08-23T17:01:21Z
dc.date.available2022-08-23T17:01:21Z
dc.date.issued2004-10-19
dc.date.submitted2009-11-23
dc.identifier.citationGenome Res. 2004 Oct;14(10B):2169-75. <a href="http://dx.doi.org/10.1101/gr.2497604">Link to article on publisher's site</a>
dc.identifier.issn1088-9051 (Print)
dc.identifier.doi10.1101/gr.2497604
dc.identifier.pmid15489340
dc.identifier.pmid15489340
dc.identifier.urihttp://hdl.handle.net/20.500.14038/43991
dc.description.abstractAn important aspect of the development of systems biology approaches in metazoans is the characterization of expression patterns of nearly all genes predicted from genome sequences. Such "localizome" maps should provide information on where (in what cells or tissues) and when (at what stage of development or under what conditions) genes are expressed. They should also indicate in what cellular compartments the corresponding proteins are localized. Caenorhabditis elegans is particularly suited for the development of a localizome map since all its 959 adult somatic cells can be visualized by microscopy, and its cell lineage has been completely described. Here we address one of the challenges of C. elegans localizome mapping projects: that of obtaining a genome-wide resource of C. elegans promoters needed to generate transgenic animals expressing localization markers such as the green fluorescent protein (GFP). To ensure high flexibility for future uses, we utilized the newly developed MultiSite Gateway system. We generated and validated "version 1.1" of the Promoterome: a resource of approximately 6000 C. elegans promoters. These promoters can be transferred easily into various Gateway Destination vectors to drive expression of markers such as GFP, alone (promoter::GFP constructs), or in fusion with protein-encoding open reading frames available in ORFeome resources (promoter::ORF::GFP).
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=15489340&dopt=Abstract">Link to Article in PubMed</a>
dc.subjectAnimals
dc.subjectAnimals, Genetically Modified
dc.subjectCaenorhabditis elegans
dc.subjectCloning, Molecular
dc.subjectGene Expression
dc.subject*Genes, Helminth
dc.subjectGreen Fluorescent Proteins
dc.subjectLuminescent Proteins
dc.subjectOpen Reading Frames
dc.subjectPromoter Regions, Genetic
dc.subjectTranscription Factors
dc.subjectGenetics and Genomics
dc.titleA first version of the Caenorhabditis elegans Promoterome
dc.typeJournal Article
dc.source.journaltitleGenome research
dc.source.volume14
dc.source.issue10B
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=1019&amp;context=pgfe_pp&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/pgfe_pp/20
dc.identifier.contextkey1070843
refterms.dateFOA2022-08-23T17:01:21Z
html.description.abstract<p>An important aspect of the development of systems biology approaches in metazoans is the characterization of expression patterns of nearly all genes predicted from genome sequences. Such "localizome" maps should provide information on where (in what cells or tissues) and when (at what stage of development or under what conditions) genes are expressed. They should also indicate in what cellular compartments the corresponding proteins are localized. Caenorhabditis elegans is particularly suited for the development of a localizome map since all its 959 adult somatic cells can be visualized by microscopy, and its cell lineage has been completely described. Here we address one of the challenges of C. elegans localizome mapping projects: that of obtaining a genome-wide resource of C. elegans promoters needed to generate transgenic animals expressing localization markers such as the green fluorescent protein (GFP). To ensure high flexibility for future uses, we utilized the newly developed MultiSite Gateway system. We generated and validated "version 1.1" of the Promoterome: a resource of approximately 6000 C. elegans promoters. These promoters can be transferred easily into various Gateway Destination vectors to drive expression of markers such as GFP, alone (promoter::GFP constructs), or in fusion with protein-encoding open reading frames available in ORFeome resources (promoter::ORF::GFP).</p>
dc.identifier.submissionpathpgfe_pp/20
dc.contributor.departmentProgram in Molecular Medicine
dc.contributor.departmentProgram in Gene Function and Expression
dc.source.pages2169-75


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