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dc.contributor.authorLandry, Benjamin D.
dc.contributor.authorDoyle, John P.
dc.contributor.authorToczyski, David P.
dc.contributor.authorBenanti, Jennifer A
dc.date2022-08-11T08:10:15.000
dc.date.accessioned2022-08-23T17:01:22Z
dc.date.available2022-08-23T17:01:22Z
dc.date.issued2012-07-26
dc.date.submitted2012-08-09
dc.identifier.citation<p>Landry BD, Doyle JP, Toczyski DP, Benanti JA (2012) F-Box Protein Specificity for G1 Cyclins Is Dictated by Subcellular Localization. PLoS Genet 8(7): e1002851. doi:10.1371/journal.pgen.1002851. <a href="http://dx.doi.org/10.1371/journal.pgen.1002851" target="_blank">Link to article on publisher's site</a></p>
dc.identifier.issn1553-7390 (Linking)
dc.identifier.doi10.1371/journal.pgen.1002851
dc.identifier.pmid22844257
dc.identifier.urihttp://hdl.handle.net/20.500.14038/43992
dc.description.abstractLevels of G1 cyclins fluctuate in response to environmental cues and couple mitotic signaling to cell cycle entry. The G1 cyclin Cln3 is a key regulator of cell size and cell cycle entry in budding yeast. Cln3 degradation is essential for proper cell cycle control; however, the mechanisms that control Cln3 degradation are largely unknown. Here we show that two SCF ubiquitin ligases, SCF(Cdc4) and SCF(Grr1), redundantly target Cln3 for degradation. While the F-box proteins (FBPs) Cdc4 and Grr1 were previously thought to target non-overlapping sets of substrates, we find that Cdc4 and Grr1 each bind to all 3 G1 cyclins in cell extracts, yet only Cln3 is redundantly targeted in vivo, due in part to its nuclear localization. The related cyclin Cln2 is cytoplasmic and exclusively targeted by Grr1. However, Cdc4 can interact with Cdk-phosphorylated Cln2 and target it for degradation when cytoplasmic Cdc4 localization is forced in vivo. These findings suggest that Cdc4 and Grr1 may share additional redundant targets and, consistent with this possibility, grr1Delta cdc4-1 cells demonstrate a CLN3-independent synergistic growth defect. Our findings demonstrate that structurally distinct FBPs are capable of interacting with some of the same substrates; however, in vivo specificity is achieved in part by subcellular localization. Additionally, the FBPs Cdc4 and Grr1 are partially redundant for proliferation and viability, likely sharing additional redundant substrates whose degradation is important for cell cycle progression.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=22844257&dopt=Abstract">Link to Article in PubMed</a>
dc.rights<p>Copyright: © 2012 Landry et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.</p>
dc.subjectF-Box Proteins
dc.subjectCyclin G1
dc.subjectGenetics and Genomics
dc.titleF-box protein specificity for g1 cyclins is dictated by subcellular localization
dc.typeJournal Article
dc.source.journaltitlePLoS genetics
dc.source.volume8
dc.source.issue7
dc.identifier.legacyfulltexthttps://escholarship.umassmed.edu/cgi/viewcontent.cgi?article=1200&amp;context=pgfe_pp&amp;unstamped=1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/pgfe_pp/200
dc.identifier.contextkey3194125
refterms.dateFOA2022-08-23T17:01:22Z
html.description.abstract<p>Levels of G1 cyclins fluctuate in response to environmental cues and couple mitotic signaling to cell cycle entry. The G1 cyclin Cln3 is a key regulator of cell size and cell cycle entry in budding yeast. Cln3 degradation is essential for proper cell cycle control; however, the mechanisms that control Cln3 degradation are largely unknown. Here we show that two SCF ubiquitin ligases, SCF(Cdc4) and SCF(Grr1), redundantly target Cln3 for degradation. While the F-box proteins (FBPs) Cdc4 and Grr1 were previously thought to target non-overlapping sets of substrates, we find that Cdc4 and Grr1 each bind to all 3 G1 cyclins in cell extracts, yet only Cln3 is redundantly targeted in vivo, due in part to its nuclear localization. The related cyclin Cln2 is cytoplasmic and exclusively targeted by Grr1. However, Cdc4 can interact with Cdk-phosphorylated Cln2 and target it for degradation when cytoplasmic Cdc4 localization is forced in vivo. These findings suggest that Cdc4 and Grr1 may share additional redundant targets and, consistent with this possibility, grr1Delta cdc4-1 cells demonstrate a CLN3-independent synergistic growth defect. Our findings demonstrate that structurally distinct FBPs are capable of interacting with some of the same substrates; however, in vivo specificity is achieved in part by subcellular localization. Additionally, the FBPs Cdc4 and Grr1 are partially redundant for proliferation and viability, likely sharing additional redundant substrates whose degradation is important for cell cycle progression.</p>
dc.identifier.submissionpathpgfe_pp/200
dc.contributor.departmentProgram in Gene Function and Expression
dc.source.pagese1002851


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