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    Non-canonical TAF complexes regulate active promoters in human embryonic stem cells

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    Authors
    Maston, Glenn A.
    Zhu, Lihua Julie
    Chamberlain, Lynn
    Lin, Ling
    Fang, Minggang
    Green, Michael R.
    UMass Chan Affiliations
    Program in Molecular Medicine
    Program in Gene Function and Expression
    Document Type
    Journal Article
    Publication Date
    2012-11-13
    Keywords
    TATA-Box Binding Protein
    TATA-Binding Protein Associated Factors
    Transcription Factors
    Transcription Factors, General
    Embryonic Stem Cells
    datasets
    Genetics and Genomics
    
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    Abstract
    The general transcription factor TFIID comprises the TATA-box-binding protein (TBP) and approximately 14 TBP-associated factors (TAFs). Here we find, unexpectedly, that undifferentiated human embryonic stem cells (hESCs) contain only six TAFs (TAFs 2, 3, 5, 6, 7 and 11), whereas following differentiation all TAFs are expressed. Directed and global chromatin immunoprecipitation analyses reveal an unprecedented promoter occupancy pattern: most active genes are bound by only TAFs 3 and 5 along with TBP, whereas the remaining active genes are bound by TBP and all six hESC TAFs. Consistent with these results, hESCs contain a previously undescribed complex comprising TAFs 2, 6, 7, 11 and TBP. Altering the composition of hESC TAFs, either by depleting TAFs that are present or ectopically expressing TAFs that are absent, results in misregulated expression of pluripotency genes and induction of differentiation. Thus, the selective expression and use of TAFs underlies the ability of hESCs to self-renew.
    Source

    Maston et al. eLife 2012;1:e00068. DOI: 10.7554/eLife.00068. Link to article on publisher's site

    DOI
    10.7554/eLife.00068
    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/44004
    PubMed ID
    23150797
    Notes

    Supplementary file is also available at http://dx.doi.org/10.7554/eLife.00068.026.

    The following datasets were generated: Maston GA, Green MR, Zhu LJ, Chamberlain L, Lin L, 2012, ChIP-chip from H9 hESCs for RNA Polymerase II, TBP, TAF3, TAF5, TAF7, and TAF11, GSE39312; http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE39312, In the public domain at GEO: http://www.ncbi.nlm.nih.gov/geo/.

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    Rights

    Copyright Maston et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.

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