Non-canonical TAF complexes regulate active promoters in human embryonic stem cells
Document Type
Journal ArticlePublication Date
2012-11-13Keywords
TATA-Box Binding ProteinTATA-Binding Protein Associated Factors
Transcription Factors
Transcription Factors, General
Embryonic Stem Cells
datasets
Genetics and Genomics
Metadata
Show full item recordAbstract
The general transcription factor TFIID comprises the TATA-box-binding protein (TBP) and approximately 14 TBP-associated factors (TAFs). Here we find, unexpectedly, that undifferentiated human embryonic stem cells (hESCs) contain only six TAFs (TAFs 2, 3, 5, 6, 7 and 11), whereas following differentiation all TAFs are expressed. Directed and global chromatin immunoprecipitation analyses reveal an unprecedented promoter occupancy pattern: most active genes are bound by only TAFs 3 and 5 along with TBP, whereas the remaining active genes are bound by TBP and all six hESC TAFs. Consistent with these results, hESCs contain a previously undescribed complex comprising TAFs 2, 6, 7, 11 and TBP. Altering the composition of hESC TAFs, either by depleting TAFs that are present or ectopically expressing TAFs that are absent, results in misregulated expression of pluripotency genes and induction of differentiation. Thus, the selective expression and use of TAFs underlies the ability of hESCs to self-renew.Source
Maston et al. eLife 2012;1:e00068. DOI: 10.7554/eLife.00068. Link to article on publisher's site
DOI
10.7554/eLife.00068Permanent Link to this Item
http://hdl.handle.net/20.500.14038/44004PubMed ID
23150797Notes
Supplementary file is also available at http://dx.doi.org/10.7554/eLife.00068.026.
The following datasets were generated: Maston GA, Green MR, Zhu LJ, Chamberlain L, Lin L, 2012, ChIP-chip from H9 hESCs for RNA Polymerase II, TBP, TAF3, TAF5, TAF7, and TAF11, GSE39312; http://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE39312, In the public domain at GEO: http://www.ncbi.nlm.nih.gov/geo/.
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Link to article in PubMedRights
Copyright Maston et al. This article is distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use and redistribution provided that the original author and source are credited.
ae974a485f413a2113503eed53cd6c53
10.7554/eLife.00068