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dc.contributor.authorSano, Makoto
dc.contributor.authorDriscoll, David R.
dc.contributor.authorDe Jesus-Monge, Wilfredo E.
dc.contributor.authorKlimstra, David S.
dc.contributor.authorLewis, Brian C.
dc.date2022-08-11T08:10:15.000
dc.date.accessioned2022-08-23T17:01:30Z
dc.date.available2022-08-23T17:01:30Z
dc.date.issued2014-01-01
dc.date.submitted2014-03-20
dc.identifier.citation<p> Sano M, Driscoll DR, De Jesus-Monge WE, Klimstra DS, Lewis BC. Activated wnt signaling in stroma contributes to development of pancreatic mucinous cystic neoplasms. Gastroenterology. 2014 Jan;146(1):257-67. doi:10.1053/j.gastro.2013.09.044. <a href="http://dx.doi.org/10.1053/j.gastro.2013.09.044" target="_blank">Link to article on publisher's site</a></p>
dc.identifier.issn0016-5085 (Linking)
dc.identifier.doi10.1053/j.gastro.2013.09.044
dc.identifier.pmid24067880
dc.identifier.urihttp://hdl.handle.net/20.500.14038/44023
dc.description.abstractBACKGROUND and AIMS: Pancreatic mucinous cystic neoplasm (MCN), a cystic tumor of the pancreas that develops most frequently in women, is a potential precursor to pancreatic ductal adenocarcinoma. MCNs develop primarily in the body and tail of the pancreas and are characterized by the presence of a mucinous epithelium and ovarian-like subepithelial stroma. We investigated the involvement of Wnt signaling in KRAS-mediated pancreatic tumorigenesis and development of MCN in mice, and Wnt activation in human MCN samples. METHODS: LSL-Kras(G12D), Ptf1a-cre mice were crossed with elastase-tva mice to allow for introduction of genes encoded by the replication-competent avian sarcoma-leukosis virus long-terminal repeat with splice acceptor viruses to pancreatic acinar cells and acinar cell progenitors, postnatally and sporadically. Repeat with splice acceptor viruses that expressed Wnt1 were delivered to the pancreatic epithelium of these mice; pancreatic lesions were analyzed by histopathology and immunohistochemical analyses. We analyzed levels of factors in Wnt signaling pathways in 19 MCN samples from patients. RESULTS: Expression of Wnt1 in the pancreatic acinar cells and acinar cell progenitors of female mice led to development of unilocular or multilocular epithelial cysts in the pancreas body and tail, similar to MCN. The cystic lesions resembled the estrogen receptor- and progesterone receptor-positive ovarian-like stroma of MCN, but lacked the typical mucinous epithelium. Activated Wnt signaling, based on nuclear localization of beta-catenin, was detected in the stroma but not cyst epithelium. Wnt signaling to beta-catenin was found to be activated in MCN samples from patients, within the ovarian-like stroma, consistent with the findings in mice. CONCLUSIONS: Based on studies of mice and pancreatic MCN samples from patients, the canonical Wnt signaling pathway becomes activated and promotes development of the ovarian-like stroma to contribute to formation of MCNs.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=24067880&dopt=Abstract">Link to Article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1053/j.gastro.2013.09.044
dc.subjectAnimals
dc.subjectCarcinoma, Pancreatic Ductal
dc.subjectCell Transformation, Neoplastic
dc.subjectCystadenocarcinoma, Mucinous
dc.subjectFemale
dc.subjectHumans
dc.subjectMice
dc.subjectMice, Transgenic
dc.subjectPancreatic Neoplasms
dc.subject*Wnt Signaling Pathway
dc.subjectWnt1 Protein
dc.subjectbeta Catenin
dc.subjectsplice acceptor
dc.subjectCancer Biology
dc.subjectDigestive System Diseases
dc.subjectGastroenterology
dc.subjectNeoplasms
dc.titleActivated wnt signaling in stroma contributes to development of pancreatic mucinous cystic neoplasms
dc.typeJournal Article
dc.source.journaltitleGastroenterology
dc.source.volume146
dc.source.issue1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/pgfe_pp/237
dc.identifier.contextkey5362383
html.description.abstract<p>BACKGROUND and AIMS: Pancreatic mucinous cystic neoplasm (MCN), a cystic tumor of the pancreas that develops most frequently in women, is a potential precursor to pancreatic ductal adenocarcinoma. MCNs develop primarily in the body and tail of the pancreas and are characterized by the presence of a mucinous epithelium and ovarian-like subepithelial stroma. We investigated the involvement of Wnt signaling in KRAS-mediated pancreatic tumorigenesis and development of MCN in mice, and Wnt activation in human MCN samples.</p> <p>METHODS: LSL-Kras(G12D), Ptf1a-cre mice were crossed with elastase-tva mice to allow for introduction of genes encoded by the replication-competent avian sarcoma-leukosis virus long-terminal repeat with splice acceptor viruses to pancreatic acinar cells and acinar cell progenitors, postnatally and sporadically. Repeat with splice acceptor viruses that expressed Wnt1 were delivered to the pancreatic epithelium of these mice; pancreatic lesions were analyzed by histopathology and immunohistochemical analyses. We analyzed levels of factors in Wnt signaling pathways in 19 MCN samples from patients.</p> <p>RESULTS: Expression of Wnt1 in the pancreatic acinar cells and acinar cell progenitors of female mice led to development of unilocular or multilocular epithelial cysts in the pancreas body and tail, similar to MCN. The cystic lesions resembled the estrogen receptor- and progesterone receptor-positive ovarian-like stroma of MCN, but lacked the typical mucinous epithelium. Activated Wnt signaling, based on nuclear localization of beta-catenin, was detected in the stroma but not cyst epithelium. Wnt signaling to beta-catenin was found to be activated in MCN samples from patients, within the ovarian-like stroma, consistent with the findings in mice.</p> <p>CONCLUSIONS: Based on studies of mice and pancreatic MCN samples from patients, the canonical Wnt signaling pathway becomes activated and promotes development of the ovarian-like stroma to contribute to formation of MCNs.</p>
dc.identifier.submissionpathpgfe_pp/237
dc.contributor.departmentProgram in Gene Function and Expression
dc.source.pages257-67


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