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dc.contributor.authorShen, Haihong
dc.contributor.authorZheng, Xuexiu
dc.contributor.authorLuecke, Stephan
dc.contributor.authorGreen, Michael R.
dc.date2022-08-11T08:10:16.000
dc.date.accessioned2022-08-23T17:01:42Z
dc.date.available2022-08-23T17:01:42Z
dc.date.issued2010-11-03
dc.date.submitted2011-04-19
dc.identifier.citationGenes Dev. 2010 Nov 1;24(21):2389-94. <a href="http://dx.doi.org/10.1101/gad.1974810">Link to article on publisher's site</a>
dc.identifier.issn0890-9369 (Linking)
dc.identifier.doi10.1101/gad.1974810
dc.identifier.pmid21041408
dc.identifier.urihttp://hdl.handle.net/20.500.14038/44063
dc.description.abstractThe U2AF35-related protein Urp has been implicated previously in splicing of the major class of U2-type introns. Here we show that Urp is also required for splicing of the minor class of U12-type introns. Urp is recruited in an ATP-dependent fashion to the U12-type intron 3' splice site, where it promotes formation of spliceosomal complexes. Remarkably, Urp also contacts the 3' splice site of a U2-type intron, but in this case is specifically required for the second step of splicing. Thus, through recognition of a common splicing element, Urp facilitates distinct steps of U2- and U12-type intron splicing.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=21041408&dopt=Abstract">Link to Article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1101/gad.1974810
dc.subjectAmino Acid Sequence
dc.subjectAnimals
dc.subjectHela Cells
dc.subjectHumans
dc.subjectImmunoblotting
dc.subjectIntrons
dc.subjectMolecular Sequence Data
dc.subjectNuclear Proteins
dc.subjectPhylogeny
dc.subjectRNA Interference
dc.subjectRNA Splice Sites
dc.subject*RNA Splicing
dc.subjectRNA, Small Nuclear
dc.subjectRibonucleoproteins
dc.subjectSpliceosomes
dc.subjectGenetics and Genomics
dc.titleThe U2AF35-related protein Urp contacts the 3' splice site to promote U12-type intron splicing and the second step of U2-type intron splicing
dc.typeJournal Article
dc.source.journaltitleGenes and development
dc.source.volume24
dc.source.issue21
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/pgfe_pp/39
dc.identifier.contextkey1946692
html.description.abstract<p>The U2AF35-related protein Urp has been implicated previously in splicing of the major class of U2-type introns. Here we show that Urp is also required for splicing of the minor class of U12-type introns. Urp is recruited in an ATP-dependent fashion to the U12-type intron 3' splice site, where it promotes formation of spliceosomal complexes. Remarkably, Urp also contacts the 3' splice site of a U2-type intron, but in this case is specifically required for the second step of splicing. Thus, through recognition of a common splicing element, Urp facilitates distinct steps of U2- and U12-type intron splicing.</p>
dc.identifier.submissionpathpgfe_pp/39
dc.contributor.departmentProgram in Molecular Medicine
dc.contributor.departmentProgram in Gene Function and Expression
dc.source.pages2389-94


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