The U2AF35-related protein Urp contacts the 3' splice site to promote U12-type intron splicing and the second step of U2-type intron splicing
dc.contributor.author | Shen, Haihong | |
dc.contributor.author | Zheng, Xuexiu | |
dc.contributor.author | Luecke, Stephan | |
dc.contributor.author | Green, Michael R. | |
dc.date | 2022-08-11T08:10:16.000 | |
dc.date.accessioned | 2022-08-23T17:01:42Z | |
dc.date.available | 2022-08-23T17:01:42Z | |
dc.date.issued | 2010-11-03 | |
dc.date.submitted | 2011-04-19 | |
dc.identifier.citation | Genes Dev. 2010 Nov 1;24(21):2389-94. <a href="http://dx.doi.org/10.1101/gad.1974810">Link to article on publisher's site</a> | |
dc.identifier.issn | 0890-9369 (Linking) | |
dc.identifier.doi | 10.1101/gad.1974810 | |
dc.identifier.pmid | 21041408 | |
dc.identifier.uri | http://hdl.handle.net/20.500.14038/44063 | |
dc.description.abstract | The U2AF35-related protein Urp has been implicated previously in splicing of the major class of U2-type introns. Here we show that Urp is also required for splicing of the minor class of U12-type introns. Urp is recruited in an ATP-dependent fashion to the U12-type intron 3' splice site, where it promotes formation of spliceosomal complexes. Remarkably, Urp also contacts the 3' splice site of a U2-type intron, but in this case is specifically required for the second step of splicing. Thus, through recognition of a common splicing element, Urp facilitates distinct steps of U2- and U12-type intron splicing. | |
dc.language.iso | en_US | |
dc.relation | <a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=21041408&dopt=Abstract">Link to Article in PubMed</a> | |
dc.relation.url | http://dx.doi.org/10.1101/gad.1974810 | |
dc.subject | Amino Acid Sequence | |
dc.subject | Animals | |
dc.subject | Hela Cells | |
dc.subject | Humans | |
dc.subject | Immunoblotting | |
dc.subject | Introns | |
dc.subject | Molecular Sequence Data | |
dc.subject | Nuclear Proteins | |
dc.subject | Phylogeny | |
dc.subject | RNA Interference | |
dc.subject | RNA Splice Sites | |
dc.subject | *RNA Splicing | |
dc.subject | RNA, Small Nuclear | |
dc.subject | Ribonucleoproteins | |
dc.subject | Spliceosomes | |
dc.subject | Genetics and Genomics | |
dc.title | The U2AF35-related protein Urp contacts the 3' splice site to promote U12-type intron splicing and the second step of U2-type intron splicing | |
dc.type | Journal Article | |
dc.source.journaltitle | Genes and development | |
dc.source.volume | 24 | |
dc.source.issue | 21 | |
dc.identifier.legacycoverpage | https://escholarship.umassmed.edu/pgfe_pp/39 | |
dc.identifier.contextkey | 1946692 | |
html.description.abstract | <p>The U2AF35-related protein Urp has been implicated previously in splicing of the major class of U2-type introns. Here we show that Urp is also required for splicing of the minor class of U12-type introns. Urp is recruited in an ATP-dependent fashion to the U12-type intron 3' splice site, where it promotes formation of spliceosomal complexes. Remarkably, Urp also contacts the 3' splice site of a U2-type intron, but in this case is specifically required for the second step of splicing. Thus, through recognition of a common splicing element, Urp facilitates distinct steps of U2- and U12-type intron splicing.</p> | |
dc.identifier.submissionpath | pgfe_pp/39 | |
dc.contributor.department | Program in Molecular Medicine | |
dc.contributor.department | Program in Gene Function and Expression | |
dc.source.pages | 2389-94 |