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dc.contributor.authorDekker, Job
dc.date2022-08-11T08:10:16.000
dc.date.accessioned2022-08-23T17:01:55Z
dc.date.available2022-08-23T17:01:55Z
dc.date.issued2008-12-12
dc.date.submitted2011-04-19
dc.identifier.citationJ Biol Chem. 2008 Dec 12;283(50):34532-40. Epub 2008 Oct 16. <a href="http://dx.doi.org/10.1074/jbc.M806479200">Link to article on publisher's site</a>
dc.identifier.issn0021-9258 (Linking)
dc.identifier.doi10.1074/jbc.M806479200
dc.identifier.pmid18930918
dc.identifier.urihttp://hdl.handle.net/20.500.14038/44112
dc.description.abstractThe higher order arrangement of nucleosomes and the level of compaction of the chromatin fiber play important roles in the control of gene expression and other genomic activities. Analysis of chromatin in vitro has suggested that under near physiological conditions chromatin fibers can become highly compact and that the level of compaction can be modulated by histone modifications. However, less is known about the organization of chromatin fibers in living cells. Here, we combine chromosome conformation capture (3C) data with distance measurements and polymer modeling to determine the in vivo mass density of a transcriptionally active 95-kb GC-rich domain on chromosome III of the yeast Saccharomyces cerevisiae. In contrast to previous reports, we find that yeast does not form a compact fiber but that chromatin is extended with a mass per unit length that is consistent with a rather loose arrangement of nucleosomes. Analysis of 3C data from a neighboring AT-rich chromosomal domain indicates that chromatin in this domain is more compact, but that mass density is still well below that of a canonical 30 nm fiber. Our approach should be widely applicable to scale 3C data to real spatial dimensions, which will facilitate the quantification of the effects of chromatin modifications and transcription on chromatin fiber organization.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=18930918&dopt=Abstract">Link to Article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1074/jbc.M806479200
dc.subjectChromatin
dc.subjectChromosomes
dc.subjectCross-Linking Reagents
dc.subjectFungal Proteins
dc.subjectModels, Statistical
dc.subjectModels, Theoretical
dc.subjectMolecular Conformation
dc.subjectNormal Distribution
dc.subjectNucleosomes
dc.subjectProtein Binding
dc.subjectProtein Conformation
dc.subjectProtein Structure, Tertiary
dc.subjectSaccharomyces cerevisiae
dc.subjectTemperature
dc.subjectTranscription, Genetic
dc.subjectGenetics and Genomics
dc.titleMapping in vivo chromatin interactions in yeast suggests an extended chromatin fiber with regional variation in compaction
dc.typeJournal Article
dc.source.journaltitleThe Journal of biological chemistry
dc.source.volume283
dc.source.issue50
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/pgfe_pp/86
dc.identifier.contextkey1946741
html.description.abstract<p>The higher order arrangement of nucleosomes and the level of compaction of the chromatin fiber play important roles in the control of gene expression and other genomic activities. Analysis of chromatin in vitro has suggested that under near physiological conditions chromatin fibers can become highly compact and that the level of compaction can be modulated by histone modifications. However, less is known about the organization of chromatin fibers in living cells. Here, we combine chromosome conformation capture (3C) data with distance measurements and polymer modeling to determine the in vivo mass density of a transcriptionally active 95-kb GC-rich domain on chromosome III of the yeast Saccharomyces cerevisiae. In contrast to previous reports, we find that yeast does not form a compact fiber but that chromatin is extended with a mass per unit length that is consistent with a rather loose arrangement of nucleosomes. Analysis of 3C data from a neighboring AT-rich chromosomal domain indicates that chromatin in this domain is more compact, but that mass density is still well below that of a canonical 30 nm fiber. Our approach should be widely applicable to scale 3C data to real spatial dimensions, which will facilitate the quantification of the effects of chromatin modifications and transcription on chromatin fiber organization.</p>
dc.identifier.submissionpathpgfe_pp/86
dc.contributor.departmentProgram in Gene Function and Expression
dc.source.pages34532-40


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