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dc.contributor.authorDostie, Josee
dc.contributor.authorZhan, Ye
dc.contributor.authorDekker, Job
dc.date2022-08-11T08:10:16.000
dc.date.accessioned2022-08-23T17:01:58Z
dc.date.available2022-08-23T17:01:58Z
dc.date.issued2007-10-12
dc.date.submitted2011-04-19
dc.identifier.citationCurr Protoc Mol Biol. 2007 Oct;Chapter 21:Unit 21.14. <a href="http://dx.doi.org/10.1002/0471142727.mb2114s80">Link to article on publisher's site</a>
dc.identifier.issn1934-3647 (Linking)
dc.identifier.doi10.1002/0471142727.mb2114s80
dc.identifier.pmid18265398
dc.identifier.urihttp://hdl.handle.net/20.500.14038/44122
dc.description.abstractChromosome conformation capture (3C) is used to quantify physical DNA contacts in vivo at high resolution. 3C was first used in yeast to map the spatial chromatin organization of chromosome III, and in higher eukaryotes to demonstrate that genomic DNA elements regulate target genes by physically interacting with them. 3C has been widely adopted for small-scale analysis of functional chromatin interactions along (cis) or between (trans) chromosomes. For larger-scale applications, chromosome conformation capture carbon copy (5C) combines 3C with ligation-mediated amplification (LMA) to simultaneously quantify hundreds of thousands of physical DNA contacts by microarray or ultra-high-throughput DNA sequencing. 5C allows the mapping of extensive networks of physical interactions among large sets of genomic elements throughout the genome. Such networks can provide important biological insights, e.g., by identifying relationships between regulatory elements and their target genes. This unit describes 5C for large-scale analysis of cis- and trans-chromatin interactions in mammalian cells.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=18265398&dopt=Abstract">Link to Article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1002/0471142727.mb2114s80
dc.subjectChromosomes
dc.subjectChromosomes, Artificial, Bacterial
dc.subjectDNA Primers
dc.subjectMolecular Biology
dc.subject*Nucleic Acid Conformation
dc.subjectOligonucleotide Array Sequence Analysis
dc.subjectPolymerase Chain Reaction
dc.subjectSequence Analysis, DNA
dc.subjectTemplates, Genetic
dc.subjectGenetics and Genomics
dc.titleChromosome conformation capture carbon copy technology
dc.typeBook Chapter
dc.source.booktitleCurrent protocols in molecular biology
dc.source.volumeChapter 21
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/pgfe_pp/95
dc.identifier.contextkey1946750
html.description.abstract<p>Chromosome conformation capture (3C) is used to quantify physical DNA contacts in vivo at high resolution. 3C was first used in yeast to map the spatial chromatin organization of chromosome III, and in higher eukaryotes to demonstrate that genomic DNA elements regulate target genes by physically interacting with them. 3C has been widely adopted for small-scale analysis of functional chromatin interactions along (cis) or between (trans) chromosomes. For larger-scale applications, chromosome conformation capture carbon copy (5C) combines 3C with ligation-mediated amplification (LMA) to simultaneously quantify hundreds of thousands of physical DNA contacts by microarray or ultra-high-throughput DNA sequencing. 5C allows the mapping of extensive networks of physical interactions among large sets of genomic elements throughout the genome. Such networks can provide important biological insights, e.g., by identifying relationships between regulatory elements and their target genes. This unit describes 5C for large-scale analysis of cis- and trans-chromatin interactions in mammalian cells.</p>
dc.identifier.submissionpathpgfe_pp/95
dc.contributor.departmentProgram in Gene Function and Expression
dc.source.pagesUnit 21.14


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