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dc.contributor.authorEthier, Michael F.
dc.contributor.authorHickler, Roger B.
dc.contributor.authorDobson, James G. Jr.
dc.date2022-08-11T08:10:16.000
dc.date.accessioned2022-08-23T17:02:11Z
dc.date.available2022-08-23T17:02:11Z
dc.date.issued1989-11-01
dc.date.submitted2008-06-12
dc.identifier.citationMech Ageing Dev. 1989 Nov;50(2):159-68.
dc.identifier.issn0047-6374 (Print)
dc.identifier.pmid2601414
dc.identifier.urihttp://hdl.handle.net/20.500.14038/44163
dc.description.abstractThe effect of in vitro age and donor age on net release of adenosine and inosine was studied in cultures of normal human fibroblasts. Confluent cultures of low-(population doubling level [PDL] 23-25) and high- (PDL 43-45) passage human lung fibroblasts derived from a 16-week-old fetal donor (IMR-90) were incubated for 30 min in physiological saline and the release of adenosine and inosine into the saline was determined by HPLC. Release of adenosine and inosine into the saline bathing low-passage human skin fibroblasts derived from a 16-week-old fetal donor (GM6111) was also determined and compared with two strains of low-passage skin fibroblasts from aged (66-67 years) donors (GM3529 and GM3524). The release of adenosine and inosine by low-passage cultures of fetal lung fibroblasts was 911 and 225 pmol/30 min per mg protein, respectively. In high-passage cultures of lung fibroblasts, release of adenosine and inosine was significantly greater at 1403 and 351 pmol/30 min per mg protein, respectively. The release of adenosine and inosine by low-passage cultures of fetal skin fibroblasts was 250 and 179 pmol/30 min per mg protein, respectively. In low-passage skin fibroblasts from aged donors, release of adenosine and inosine was significantly greater at 583 and 652 pmol/30 min per mg protein, respectively. These results indicate that the net release of adenosine and inosine by cultured human fibroblasts into their extracellular environment is enhanced by in vitro aging of lung fibroblasts and is greater in skin fibroblast from aged donors.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?cmd=Retrieve&db=PubMed&list_uids=2601414&dopt=Abstract ">Link to article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1016/0047-6374(89)90011-0
dc.subjectAdenosine
dc.subjectAging
dc.subjectCells, Cultured
dc.subjectFibroblasts
dc.subjectHumans
dc.subjectInosine
dc.subjectComparative and Evolutionary Physiology
dc.subjectPhysiology
dc.titleAging increases adenosine and inosine release by human fibroblast cultures
dc.typeJournal Article
dc.source.journaltitleMechanisms of ageing and development
dc.source.volume50
dc.source.issue2
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/physio_pp/45
dc.identifier.contextkey523460
html.description.abstract<p>The effect of in vitro age and donor age on net release of adenosine and inosine was studied in cultures of normal human fibroblasts. Confluent cultures of low-(population doubling level [PDL] 23-25) and high- (PDL 43-45) passage human lung fibroblasts derived from a 16-week-old fetal donor (IMR-90) were incubated for 30 min in physiological saline and the release of adenosine and inosine into the saline was determined by HPLC. Release of adenosine and inosine into the saline bathing low-passage human skin fibroblasts derived from a 16-week-old fetal donor (GM6111) was also determined and compared with two strains of low-passage skin fibroblasts from aged (66-67 years) donors (GM3529 and GM3524). The release of adenosine and inosine by low-passage cultures of fetal lung fibroblasts was 911 and 225 pmol/30 min per mg protein, respectively. In high-passage cultures of lung fibroblasts, release of adenosine and inosine was significantly greater at 1403 and 351 pmol/30 min per mg protein, respectively. The release of adenosine and inosine by low-passage cultures of fetal skin fibroblasts was 250 and 179 pmol/30 min per mg protein, respectively. In low-passage skin fibroblasts from aged donors, release of adenosine and inosine was significantly greater at 583 and 652 pmol/30 min per mg protein, respectively. These results indicate that the net release of adenosine and inosine by cultured human fibroblasts into their extracellular environment is enhanced by in vitro aging of lung fibroblasts and is greater in skin fibroblast from aged donors.</p>
dc.identifier.submissionpathphysio_pp/45
dc.contributor.departmentDepartment of Medicine, Division of Pulmonary, Allergy and Critical Care
dc.contributor.departmentDepartment of Physiology
dc.source.pages159-68


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