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    ATP as a source of interstitial adenosine in the rat heart

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    Authors
    Lorbar, Mojca
    Fenton, Richard A.
    Dobson, James G.
    UMass Chan Affiliations
    Department of Physiology
    Document Type
    Journal Article
    Publication Date
    1999-10-30
    Keywords
    Adenosine
    Adenosine Triphosphate
    Animals
    Calcium
    Male
    Myocardial Contraction
    Myocardium
    Norepinephrine
    Perfusion
    Rats
    Rats, Sprague-Dawley
    Stellate Ganglion
    Physiology
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    Link to Full Text
    http://dx.doi.org/10.1139/cjpp-77-8-579
    Abstract
    The contribution of neuronal ATP to interstitial adenosine levels was investigated in isolated perfused rat hearts. Ventricular surface transudates, representing interstitial fluid, were analyzed for norepinephrine, ATP, and adenosine. Exocytotic release of norepinephrine was induced by electrical stimulation of cardiac efferents emanating from the stellate ganglion. Ganglion stimulation increased contractility, interstitial norepinephrine, ATP, and adenosine. Interstitial adenosine was 11- to 27-fold higher than interstitial ATP, suggesting that the released ATP is unlikely the only source of adenosine. In the presence of AOPCP (alpha,beta-methyleneadenosine 5'-diphosphate), an ecto-5'-nucleotidase inhibitor, the ganglion-stimulated increase in interstitial ATP and adenosine reached levels similar to those in the absence of AOPCP, also suggesting that adenosine does not derive from extracellular ATP. The perfusate Ca2+ was raised from 1 to 4 mM to determine the importance of the enhanced contractile function on the levels of norepinephrine, ATP, and adenosine. The results were increases in contractility and interstitial norepinephrine, ATP, and adenosine, which were not suppressed with atenolol, indicating a norepinephrine-independent release of ATP and adenosine. Reserpine treatment and administration of guanethidine depleted the catecholamine stores and diminished the catecholamine release, respectively. However, neither agent altered Ca2+-induced increases in ATP and adenosine. It is concluded that the amount of neuronal-derived ATP is low and most likely does not contribute significantly to interstitial levels of adenosine. Furthermore, elevations in interstitial norepinephrine, ATP, and adenosine are associated with neuronal-independent increases in contractile function.
    Source
    Can J Physiol Pharmacol. 1999 Aug;77(8):579-88.
    Permanent Link to this Item
    http://hdl.handle.net/20.500.14038/44175
    PubMed ID
    10543721
    Related Resources
    Link to article in PubMed
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    UMass Chan Faculty and Researcher Publications

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