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dc.contributor.authorWang, Zhenyu
dc.contributor.authorShi, Yuqin
dc.contributor.authorMa, Suheng
dc.contributor.authorHuang, Qian
dc.contributor.authorYap, Yi Tian
dc.contributor.authorShi, Lin
dc.contributor.authorZhang, Shiyang
dc.contributor.authorZhou, Ting
dc.contributor.authorLi, Wei
dc.contributor.authorHu, Bo
dc.contributor.authorZhang, Ling
dc.contributor.authorKrawetz, Stephen A.
dc.contributor.authorPazour, Gregory J.
dc.contributor.authorHess, Rex A.
dc.contributor.authorZhang, Zhibing
dc.date2022-08-11T08:10:18.000
dc.date.accessioned2022-08-23T17:03:19Z
dc.date.available2022-08-23T17:03:19Z
dc.date.issued2020-01-01
dc.date.submitted2020-02-06
dc.identifier.citation<p>Wang Z, Shi Y, Ma S, Huang Q, Yap YT, Shi L, Zhang S, Zhou T, Li W, Hu B, Zhang L, Krawetz SA, Pazour GJ, Hess RA, Zhang Z. Abnormal fertility, acrosome formation, IFT20 expression and localization in conditional <em>Gmap210</em> knockout mice. <em>Am J Physiol Cell Physiol</em>. 2020 Jan 1;318(1):C174-C190. doi: 10.1152/ajpcell.00517.2018. Epub 2019 Oct 2. PMID: 31577511; PMCID: PMC6985835. <a href="https://doi.org/10.1152/ajpcell.00517.2018">Link to article on publisher's site</a></p>
dc.identifier.issn0363-6143 (Linking)
dc.identifier.doi10.1152/ajpcell.00517.2018
dc.identifier.pmid31577511
dc.identifier.urihttp://hdl.handle.net/20.500.14038/44400
dc.description.abstractGMAP210 (TRIP11) is a cis-Golgi network-associated protein and a Golgi membrane receptor for IFT20, an intraflagellar transport component essential for male fertility and spermiogenesis in mice. To investigate the role of GMAP210 in male fertility and spermatogenesis, floxed Gmap210 mice were bred with Stra8-iCre mice so that the Gmap210 gene is disrupted in spermatocytes and spermatids in this study. The Gmap210(flox/flox): Stra8-iCre mutant mice showed no gross abnormalities and survived to adulthood. In adult males, testis and body weights showed no difference between controls and mutant mice. Low-magnification histological examination of the testes revealed normal seminiferous tubule structure, but sperm counts and fertility were significantly reduced in mutant mice compared with controls. Higher resolution examination of the mutant seminiferous epithelium showed that nearly all sperm had more oblong, abnormally shaped heads, while the sperm tails appeared to have normal morphology. Electron microscopy also revealed abnormally shaped sperm heads but normal axoneme core structure; some sperm showed membrane defects in the midpiece. In mutant mice, expression levels of IFT20 and other selective acrosomal proteins were significantly reduced, and their localization was also affected. Peanut-lectin, an acrosome maker, was almost absent in the spermatids and epididymal sperm. Mitochondrion staining was highly concentrated in the heads of sperm, suggesting that the midpieces were coiling around or aggregating near the heads. Defects in acrosome biogenesis were further confirmed by electron microscopy. Collectively, our findings suggest that GMAP210 is essential for acrosome biogenesis, normal mitochondrial sheath formation, and male fertility, and it determines expression levels and acrosomal localization of IFT20 and other acrosomal proteins.
dc.language.isoen_US
dc.relation<p><a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=31577511&dopt=Abstract">Link to Article in PubMed</a></p>
dc.relation.urlhttps://doi.org/10.1152/ajpcell.00517.2018
dc.subjectGMAP210
dc.subjectIFT20
dc.subjectTrip11
dc.subjectacrosome formation
dc.subjectmale fertility
dc.subjectBiochemistry, Biophysics, and Structural Biology
dc.subjectCellular and Molecular Physiology
dc.subjectDevelopmental Biology
dc.titleAbnormal fertility, acrosome formation, IFT20 expression and localization in conditional Gmap210 knockout mice
dc.typeJournal Article
dc.source.journaltitleAmerican journal of physiology. Cell physiology
dc.source.volume318
dc.source.issue1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/pmm_pp/134
dc.identifier.contextkey16469695
html.description.abstract<p>GMAP210 (TRIP11) is a cis-Golgi network-associated protein and a Golgi membrane receptor for IFT20, an intraflagellar transport component essential for male fertility and spermiogenesis in mice. To investigate the role of GMAP210 in male fertility and spermatogenesis, floxed Gmap210 mice were bred with Stra8-iCre mice so that the Gmap210 gene is disrupted in spermatocytes and spermatids in this study. The Gmap210(flox/flox): Stra8-iCre mutant mice showed no gross abnormalities and survived to adulthood. In adult males, testis and body weights showed no difference between controls and mutant mice. Low-magnification histological examination of the testes revealed normal seminiferous tubule structure, but sperm counts and fertility were significantly reduced in mutant mice compared with controls. Higher resolution examination of the mutant seminiferous epithelium showed that nearly all sperm had more oblong, abnormally shaped heads, while the sperm tails appeared to have normal morphology. Electron microscopy also revealed abnormally shaped sperm heads but normal axoneme core structure; some sperm showed membrane defects in the midpiece. In mutant mice, expression levels of IFT20 and other selective acrosomal proteins were significantly reduced, and their localization was also affected. Peanut-lectin, an acrosome maker, was almost absent in the spermatids and epididymal sperm. Mitochondrion staining was highly concentrated in the heads of sperm, suggesting that the midpieces were coiling around or aggregating near the heads. Defects in acrosome biogenesis were further confirmed by electron microscopy. Collectively, our findings suggest that GMAP210 is essential for acrosome biogenesis, normal mitochondrial sheath formation, and male fertility, and it determines expression levels and acrosomal localization of IFT20 and other acrosomal proteins.</p>
dc.identifier.submissionpathpmm_pp/134
dc.contributor.departmentProgram in Molecular Medicine
dc.source.pagesC174-C190


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