The nuclear basket proteins Mlp1p and Mlp2p are part of a dynamic interactome including Esc1p and the proteasome
Authors
Niepel, MarioMolloy, Kelly R.
Williams, Rosemary
Farr, Julia C.
Meinema, Anne C.
Vecchietti, Nicholas
Cristea, Ileana M.
Chait, Brian T.
Rout, Michael P.
Strambio-De-Castillia, Caterina
UMass Chan Affiliations
Program in Molecular MedicineDocument Type
Journal ArticlePublication Date
2013-12-01Keywords
nuclear pore complexNPC
Mlp1p
Mlp2p
messenger ribonucleoproteins
mRNPs
Amino Acids, Peptides, and Proteins
Biochemistry, Biophysics, and Structural Biology
Cell Biology
Enzymes and Coenzymes
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Show full item recordAbstract
The basket of the nuclear pore complex (NPC) is generally depicted as a discrete structure of eight protein filaments that protrude into the nucleoplasm and converge in a ring distal to the NPC. We show that the yeast proteins Mlp1p and Mlp2p are necessary components of the nuclear basket and that they also embed the NPC within a dynamic protein network, whose extended interactome includes the spindle organizer, silencing factors, the proteasome, and key components of messenger ribonucleoproteins (mRNPs). Ultrastructural observations indicate that the basket reduces chromatin crowding around the central transporter of the NPC and might function as a docking site for mRNP during nuclear export. In addition, we show that the Mlps contribute to NPC positioning, nuclear stability, and nuclear envelope morphology. Our results suggest that the Mlps are multifunctional proteins linking the nuclear transport channel to multiple macromolecular complexes involved in the regulation of gene expression and chromatin maintenance.Source
Niepel M, Molloy KR, Williams R, Farr JC, Meinema AC, Vecchietti N, Cristea IM, Chait BT, Rout MP, Strambio-De-Castillia C. The nuclear basket proteins Mlp1p and Mlp2p are part of a dynamic interactome including Esc1p and the proteasome. Mol Biol Cell. 2013 Dec;24(24):3920-38. doi: 10.1091/mbc.E13-07-0412. Epub 2013 Oct 23. PMID: 24152732; PMCID: PMC3861087. Link to article on publisher's site
DOI
10.1091/mbc.E13-07-0412Permanent Link to this Item
http://hdl.handle.net/20.500.14038/44421PubMed ID
24152732Related Resources
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© 2013 Niepel et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).Distribution License
http://creativecommons.org/licenses/by-nc-sa/3.0/ae974a485f413a2113503eed53cd6c53
10.1091/mbc.E13-07-0412
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Except where otherwise noted, this item's license is described as © 2013 Niepel et al. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/by-nc-sa/3.0).