Circulating microRNA profiles in human patients with acetaminophen hepatotoxicity or ischemic hepatitis
Lee, Rosalind C.
McGill, Mitchell R.
Curry, Steven C.
Ambros, Victor R.
UMass Chan AffiliationsDepartment of Medicine
RNA Therapeutics Institute
Program in Molecular Medicine
Document TypeJournal Article
Real-Time Polymerase Chain Reaction
Digestive System Diseases
MetadataShow full item record
AbstractWe have identified, by quantitative real-time PCR, hundreds of miRNAs that are dramatically elevated in the plasma or serum of acetaminophen (APAP) overdose patients. Most of these circulating microRNAs decrease toward normal levels during treatment with N-acetyl cysteine (NAC). We identified a set of 11 miRNAs whose profiles and dynamics in the circulation during NAC treatment can discriminate APAP hepatotoxicity from ischemic hepatitis. The elevation of certain miRNAs can precede the dramatic rise in the standard biomarker, alanine aminotransferase (ALT), and these miRNAs also respond more rapidly than ALT to successful treatment. Our results suggest that miRNAs can serve as sensitive diagnostic and prognostic clinical tools for severe liver injury and could be useful for monitoring drug-induced liver injury during drug discovery.
SourceProc Natl Acad Sci U S A. 2014 Aug 19;111(33):12169-74. doi: 10.1073/pnas.1412608111. Epub 2014 Aug 4. Link to article on publisher's site
Permanent Link to this Itemhttp://hdl.handle.net/20.500.14038/44425
Related ResourcesLink to Article in PubMed