Circulating microRNA profiles in human patients with acetaminophen hepatotoxicity or ischemic hepatitis
Authors
Ward, JeanineKanchagar, Chitra
Veksler-Lublinsky, Isana
Lee, Rosalind C.
McGill, Mitchell R.
Jaeschke, Hartmut
Curry, Steven C.
Ambros, Victor R.
UMass Chan Affiliations
Department of MedicineRNA Therapeutics Institute
Program in Molecular Medicine
Document Type
Journal ArticlePublication Date
2014-08-19Keywords
AcetaminophenAcetylcysteine
Alanine Transaminase
Hepatitis
Humans
Ischemia
MicroRNAs
Poisoning
Real-Time Polymerase Chain Reaction
Biochemistry
Digestive System Diseases
Hepatology
Molecular Biology
Molecular Genetics
Metadata
Show full item recordAbstract
We have identified, by quantitative real-time PCR, hundreds of miRNAs that are dramatically elevated in the plasma or serum of acetaminophen (APAP) overdose patients. Most of these circulating microRNAs decrease toward normal levels during treatment with N-acetyl cysteine (NAC). We identified a set of 11 miRNAs whose profiles and dynamics in the circulation during NAC treatment can discriminate APAP hepatotoxicity from ischemic hepatitis. The elevation of certain miRNAs can precede the dramatic rise in the standard biomarker, alanine aminotransferase (ALT), and these miRNAs also respond more rapidly than ALT to successful treatment. Our results suggest that miRNAs can serve as sensitive diagnostic and prognostic clinical tools for severe liver injury and could be useful for monitoring drug-induced liver injury during drug discovery.Source
Proc Natl Acad Sci U S A. 2014 Aug 19;111(33):12169-74. doi: 10.1073/pnas.1412608111. Epub 2014 Aug 4. Link to article on publisher's siteDOI
10.1073/pnas.1412608111Permanent Link to this Item
http://hdl.handle.net/20.500.14038/44425PubMed ID
25092309Related Resources
Link to Article in PubMedae974a485f413a2113503eed53cd6c53
10.1073/pnas.1412608111