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dc.contributor.authorElewa, Ahmed M.
dc.contributor.authorShirayama, Masaki
dc.contributor.authorKaymak, Ebru
dc.contributor.authorHarrison, Paul F.
dc.contributor.authorPowell, David R.
dc.contributor.authorDu, Zhuo
dc.contributor.authorChute, Christopher D.
dc.contributor.authorWoolf, Hannah
dc.contributor.authorYi, Dongni
dc.contributor.authorIshidate, Takao
dc.contributor.authorSrinivasan, Jagan
dc.contributor.authorBao, Zhirong
dc.contributor.authorBeilharz, Traude H.
dc.contributor.authorRyder, Sean P.
dc.contributor.authorMello, Craig C.
dc.date2022-08-11T08:10:18.000
dc.date.accessioned2022-08-23T17:03:37Z
dc.date.available2022-08-23T17:03:37Z
dc.date.issued2015-07-06
dc.date.submitted2016-04-13
dc.identifier.citationDev Cell. 2015 Jul 6;34(1):108-18. doi: 10.1016/j.devcel.2015.05.024. Epub 2015 Jun 18. <a href="http://dx.doi.org/10.1016/j.devcel.2015.05.024">Link to article on publisher's site</a>
dc.identifier.issn1534-5807 (Linking)
dc.identifier.doi10.1016/j.devcel.2015.05.024
dc.identifier.pmid26096734
dc.identifier.urihttp://hdl.handle.net/20.500.14038/44464
dc.description.abstractThe regulation of mRNA translation is of fundamental importance in biological mechanisms ranging from embryonic axis specification to the formation of long-term memory. POS-1 is one of several CCCH zinc-finger RNA-binding proteins that regulate cell fate specification during C. elegans embryogenesis. Paradoxically, pos-1 mutants exhibit striking defects in endo-mesoderm development but have wild-type distributions of SKN-1, a key determinant of endo-mesoderm fates. RNAi screens for pos-1 suppressors identified genes encoding the cytoplasmic poly(A)-polymerase homolog GLD-2, the Bicaudal-C homolog GLD-3, and the protein NEG-1. We show that NEG-1 localizes in anterior nuclei, where it negatively regulates endo-mesoderm fates. In posterior cells, POS-1 binds the neg-1 3' UTR to oppose GLD-2 and GLD-3 activities that promote NEG-1 expression and cytoplasmic lengthening of the neg-1 mRNA poly(A) tail. Our findings uncover an intricate series of post-transcriptional regulatory interactions that, together, achieve precise spatial expression of endo-mesoderm fates in C. elegans embryos.
dc.language.isoen_US
dc.relation<a href="http://www.ncbi.nlm.nih.gov/entrez/query.fcgi?db=pubmed&cmd=Retrieve&list_uids=26096734&dopt=Abstract">Link to Article in PubMed</a>
dc.relation.urlhttp://dx.doi.org/10.1016/j.devcel.2015.05.024
dc.subjectAnimals
dc.subjectCaenorhabditis elegans
dc.subjectCaenorhabditis elegans Proteins
dc.subjectCarrier Proteins
dc.subjectCell Differentiation
dc.subjectCytoplasm
dc.subjectGene Expression Regulation, Developmental
dc.subjectGerm Cells
dc.subjectMesoderm
dc.subjectNuclear Proteins
dc.subjectPolyadenylation
dc.subjectRNA, Helminth
dc.subjectRNA, Messenger
dc.subjectCell Biology
dc.subjectDevelopmental Biology
dc.subjectMolecular Biology
dc.titlePOS-1 Promotes Endo-mesoderm Development by Inhibiting the Cytoplasmic Polyadenylation of neg-1 mRNA
dc.typeJournal Article
dc.source.journaltitleDevelopmental cell
dc.source.volume34
dc.source.issue1
dc.identifier.legacycoverpagehttps://escholarship.umassmed.edu/pmm_pp/58
dc.identifier.contextkey8471542
html.description.abstract<p>The regulation of mRNA translation is of fundamental importance in biological mechanisms ranging from embryonic axis specification to the formation of long-term memory. POS-1 is one of several CCCH zinc-finger RNA-binding proteins that regulate cell fate specification during C. elegans embryogenesis. Paradoxically, pos-1 mutants exhibit striking defects in endo-mesoderm development but have wild-type distributions of SKN-1, a key determinant of endo-mesoderm fates. RNAi screens for pos-1 suppressors identified genes encoding the cytoplasmic poly(A)-polymerase homolog GLD-2, the Bicaudal-C homolog GLD-3, and the protein NEG-1. We show that NEG-1 localizes in anterior nuclei, where it negatively regulates endo-mesoderm fates. In posterior cells, POS-1 binds the neg-1 3' UTR to oppose GLD-2 and GLD-3 activities that promote NEG-1 expression and cytoplasmic lengthening of the neg-1 mRNA poly(A) tail. Our findings uncover an intricate series of post-transcriptional regulatory interactions that, together, achieve precise spatial expression of endo-mesoderm fates in C. elegans embryos.</p>
dc.identifier.submissionpathpmm_pp/58
dc.contributor.departmentDepartment of Biochemistry and Molecular Pharmacology
dc.contributor.departmentRNA Therapeutics Institute
dc.contributor.departmentProgram in Molecular Medicine
dc.source.pages108-18


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