Preparation and characterization of yeast cell wall beta-glucan encapsulated humic acid nanoparticles as an enhanced aflatoxin B1 binder
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Authors
Hamza, ZeinabEl-Hashash, Maher
Aly, Soher
Hathout, Amal
Soto, Ernesto
Sabry, Bassem
Ostroff, Gary R.
UMass Chan Affiliations
Program in Molecular MedicineDocument Type
Journal ArticlePublication Date
2019-01-01Keywords
Aflatoxin B(1) (AFB(1))Glucan Mannan Lipid Particle (GMLP)
Humic acid (HA)
In vitro gastrointestinal models
Stability and cytotoxicity
Yeast cell wall (YCW)
Biochemistry
Medicinal-Pharmaceutical Chemistry
Molecular Biology
Polymer Chemistry
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Show full item recordAbstract
This study aimed to assess the effect of encapsulating humic acid inside yeast cell walls (YCW) to detoxify AFB1 in in vitro gastrointestinal models. Glucan Mannan Lipid Particles (GMLPs) from Saccharomyces cerevisiae cell walls showed the highest AFB1 adsorption in simulated gastric fluid (SGF) after 10 min, and in simulated intestinal fluid (SIF) after 1 h. GMLPs are hollow 3-4 micron porous microspheres that provide an efficient system for the synthesis and encapsulation of AFB1-absorbing nanoparticles (NPs). Humic acid nanoparticles (HA-NPs) were synthesized within the GMLP cavity by complexation with ferric chloride. Encapsulating HA-NPs in GMLPs increased HA-NP stability in SIF. The hybrid GMLP HA-NP formulation synergistically enhanced AFB1 binding compared to individual GMLP and HA components in SGF and in SIF. Cytotoxicity on a murine macrophage cell line demonstrated that GMLP HA-NP-AFB1 complexes were stable in both SGF and SIF, detoxified AFB1 and are suitable for in vivo testing.Source
Carbohydr Polym. 2019 Jan 1;203:185-192. doi: 10.1016/j.carbpol.2018.08.047. Epub 2018 Aug 12. Link to article on publisher's site
DOI
10.1016/j.carbpol.2018.08.047Permanent Link to this Item
http://hdl.handle.net/20.500.14038/44500PubMed ID
30318202Related Resources
ae974a485f413a2113503eed53cd6c53
10.1016/j.carbpol.2018.08.047